Treatment Of Spinal Cord Injury In Rats With Granulocyte-Colony Stimulating Factor:an Experimental Study

ObjectiveTo observe the therapeutic efficacy of Granulocyte-Colony Stimulating Factor(G-CSF)in rats with spinal cord injury.MethodsAllen’s（WD）spinal cord injury rat model was constructed. Forty adult healthy Wistarrats were divided into randomly2groups: control group and G-CSF treatmentgroup,Twenty rats per group. G-CSF treatment group:Subcutaneous injectionG-CSF(50μg/kg/day;diluted with physiological saline into20μg/ml)on5consecutivedays after injuried. Control group: Given an equal volume of physiological salineinjection after injuried,method of injection same with treatment group.The rats wereterminated at the24thhour，the72thhour and on the7thday，the14thday，the21thdayafter trauma(Four rats at a time). The pathomorphology and apoptosis of the injuriedsite of the spinal cord were observated,discern at1th，3th，7th，14th，21thday commitmotor function score(Gales score)in that the recovered level of neurological functionof posterior limb in rats.ResultsThe group treated with G-CSF after spinal cord injury were compared with controlgroup,significantly enhanced ethological score，pathomorphology and apoptosisdetection（P <0.05）. ConclusionSubcutaneous injection G-CSF can play the repair and protection role in the spinalnerve through the reduction of the neuronal cell apoptosis in rats after spinal cordinjury.