Study On The Role Of MRP1and MRP4in Severe Preeclampsia

Background and Objective Preeclampsia (PE) is a common complication of pregnancy-specific. Severe preeclampsia can seriously endanger the health of both the pregnant women and the fetus, while the etiology and pathogenesis of the disease are still not clear. It is consider that, in normal pregnancy, the immune status of both maternal-fetal interface and peripheral are characterized by cell immune-deficiency and humoral dominant, however, in preeclampsia, the immune environment is biased in favor of the cellular immune status. The mechanisms for regulation of the normal pregnancy and preeclampsia in vivo immune environment orientation are not yet clear. Recent studies have found that multidrug resistance-associated protein (MRP) transporter protein involved in the regulation of the immune response in the body, and had regulatory function on immune cells. Currently, the research on the relationship between MRP land MRP4and the pathogenesis of preeclampsia has not been reported at home and abroad. This research through a comparative study of MRP1, MRP4and their transport substrate leukotriene B4(LTB4) between normal pregnancy and severe preeclampsia, in order to explore the role of MRP1and MRP4in pathogenesis of preeclampsia, and provide new ideas to further elucidated the pathogenesis of preeclampsia.Methods We examined the mRNA expression level of MRP1and MRP4in maternal-fetal interface (placenta and deciduas) in normal pregnant women during different stages and in severe preeclampsia patients during the third trimester by Real-time quantitative RT-PCR method and compared the MRP1and MRP4mRNA expression difference between normal pregnancy and severe preeclampsia. The ELISA double antibody sandwich method was used to detect the LTB4concentration in serum in healthy non-pregnant women, normal pregnant women and patients with severe preeclampsia, and its expression changes was compared between pre-pregnant, pregnancy various periods and severe preeclampsia patients. Results1. MRP1and MRP4mRNA expression in tissue of placenta and deciduas Compared to normal late pregnant group, the expression of MRP1and MRP4mRNA in placenta significantly increased in the severe preeclampsia group (P <0.05); compared to both normal early and late pregnant group, the expression of MRP1and MRP4mRNA in deciduas obviously elevated in the severe preeclampsia group (P<0.05); while there was no significant difference in deciduas MRP1and MRP4expression in normal early pregnant group and normal late pregnant group (P>0.05).2. Serum LTB4expression (1) There were significantly differences in the concentration of LTB4in the serum during pre-pregnant and different stages of healthy pregnancy. After pregnancy, the concentration of LTB4in the serum of normal women declined. Compared to the pre-pregnant group, the early pregnant group, and third trimester group, the LTB4concentration markedly dropped off in second trimester group [33.31(20.78-84.37) vs.53.31(21.81-159.45), P<0.05;33.31(20.78-84.37) vs.53.17(22.53-146.62), P<0.01;33.31(20.78-84.37) vs.37.44(22.70-154.86), P<0.05]; compared to the pre-pregnant group, the concentration of LTB4in serum slightly decreased in early pregnancy group [53.17(22.53-146.62) vs.53.31(21.81-159.45), P>0.05]; compared to both pre-pregnant and the early pregnant group, the LTB4level slightly reduced in third trimester group [37.44(22.70-154.86)vs.53.31(21.81-159.45), P>0.05;37.44(22.70-154.86) vs.53.17(22.53-146.62), P>0.05];(2) There was no significant difference in that the serum LTB4expression in severe preeclampsia group and gestational age-matched normal pregnant control group [37.43(19.34-85.99) vs.37.43(27.06-132.55), P>0.05].Conclusion1. Both MRP1and MRP4mRNA express in deciduas in early and late pregnancy, and also express in placenta in late pregnancy.2. Compare to the normal pregnancies, the mRNA expression of MRP1and MRP4in tissue of placenta and deciduas significantly increased in severe preeclampsia patients, which maybe involved in the pathogenesis of preeclampsia. 3. There were significantly differences in the concentration of LTB4in the serum during pre-pregnant and different stages of healthy pregnancy. The serum LTB4expression changes may play an important role in the success and maintenance of normal pregnancy. However, the serum LTB4expression changes maybe play an insignificant role in the pathogenesis of preeclampsia.