Effect Of Wenqingyin On Expression Of Epidermal Terminal Differentiation Protein FLG In Skin Epidermis Differentiation Proteins

Part1Effect of WQY complex prescription on proliferation anddifferentiation of keratinocyte in vivoObjective: To explore the mechanism of Wenqingyin complexprescription on treating psoriasis by investigate the effect to keratinocyteproliferation and differentiation.Methods： Cultured keratinocyte cell lines HaCaT in differentconcentration of WQY in vivo. Observed the HaCaT morphologic changethrough inverted microscope. Learned the effect of WQY for the growth ofHaCaT through CCK-8assay. Studied the change of the expression of FLGin HaCaT after treating with WQY for48h by semiquantitative RT-PCR.Results：(1)HaCaT proliferation could be inhibited with differentconcentration of WQY. The minimum inhibitory concentration is5mg/ml, andwith the increasing of concentration,the inhibitory effect was enhanced(P＜0.05).(2)Treatment with10mg/ml、20mg/ml、50mg/ml WQY in HaCaT for48h,the expression of filaggrin mRNA were significantly higher than thenormal group. The groups of different concentration of WQY also had thesignificant difference(P＜0.01).Conclusions: Not only WQY can inhibit the HaCaT proliferation,butalso can promote the HaCaT differentiation. Which imply that WQY mayhave an effect for therapy of psoriasis. Part2Effects of the cream of WQY on2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions inmiceObjective: To explore the establishment of AD model by DNCBsensitization on mice and to study the therapeutic effect of the cream of WQYon the animal model of atopic dermatitis mice.Methods: Atopic dermatitis-like animal model was established by topictreatment with5%2,4-nitrochlorobenzene on the hair removed back of mice.Compaired the treatment with WQY(the treatment with Halometasone creamas the therapeutic control group)with the normal control group, and observedthe change of histopathology. Detected the expression of FLG among thetherapeutic groups, the AD-like animal model group and the normal controlgroup through immunohistochemical staining.Results：(1)We observed the changes of lesion and histopathologycondition of those groups: normal control group skin appeared almostcompletely normal, but histopathology showed slightly keratosis; AD-likeanimal group lesion appeared erythema，exudation，crusting，bleeding andother manifestations. Histopathology showed hyperkeratosis，parakeratosis，acanthosis, mild spongy edema, dilated capillary vessel with infiltration alarge number of lymphocytes both in superficial and medical layer of derma;WQY group lesion appeared that erythema and exudation were lighten thanAD-like animal group. Histopathology showed slightly hyperkeratosis andspongy edema, dilated capillary vessel with infiltration a small amount oflymphocytes in derma.(2)Expression of FLG were discovered both in the lesion of AD-like model group and normal control group, but model groupwas significant higher than control group(1.8±0.262和4.8±0.185).Expressionof FLG WQY and Halometasone therapeutic groups were close to the normalcontrol group.Conclusions：The AD mice model was successfully established byDNCB sensitization in mice. WQY treatment could up-regulate the expressionof FLG then inhibit the skin lesion, which was probably good for the therapyof clinical AD.