Extracting Of Total Flavonoids In Lycium Chinensis Leaves And Its Effect On Learning Memory Of Mice Exposed To Lead

Modern natural medicine research showed that, total flavonoids in leaves of Lycium barbarum is the effective ingredient of the medicine Chinese wolfberry leaves. The total flavonoids in natural medicine research focused in recent years, the research shows that it has a strong biological activity, such as anti-aging, antioxidant, antibacterial and antiviral, lowering blood lipid, inhibiting the growth of tumor and other efficacy. With the rapid development of industry, the survival of the human environment is facing the threat of various pollutants, including heavy metals of lead pollution is particularly serious. The nerve poison effect and mechanism of heavy metal lead on the human body has many years of research history, lead drugs have been developed for the treatment of lead poisoning, but for the total flavonoids from Lycium leaf compounds poisoning recovery was few reports on the damage of lead. Use mouse as the experimental object, intraperitoneal injection of lead acetate to establish model of memory impairment, the total flavonoids from Lycium leaf extract gastric perfusion, function change of experimental research on learning and memory in mice through the Morris water maze test analysis system and immunohistochemistry, at the same time, blood serum was separated to study its effect on the level of free radical in mice.Abstract:[Objective]:Lycium chinensis leaves flavonoids on learning and memory repair and protective effects on mice exposed to lead acetate, affect the metabolism of free radical and Bcl-2expression.[Methods]: exposure to lead acetate through the establishment of model mice by intraperitoneal injection of40mg/kg, daily according to the high, medium and low (50mg/kg,100mg/kg,200mg/kg) three concentration levels were administered flavonoids gastric Lycium chinensis leaves, Morris water maze spatial learning and memory training and testing was started after6weeks of intragastric administration, test the SOD and MDA level in brain tissue in mice and take pictures to the hippocampus by Bcl-2immunohistochemistry.[results]:water maze test results show that the concentration of total flavonoids medium (1OOmg/kg) and high concentration group (200mg/kg) mice escape latency was significantly less than the control mice to lead acetate; SOD level in medium total flavonoids group was significantly higher than that in lead acetate control group (P<0.05); medium concentration and high concentration group of MDA level (P<0.05) was significantly lower than that in lead acetate control group; the number of positive cells of the mouse dentate gyrus Bcl-2immune group, total flavonoids high concentration group of Bcl-2positive cell number was significantly lower than that of lead acetate model group (P<0.05) of Bcl-2positive cell number.[Conclusion]:intragastric administration of certain concentration (100mg/kg,200mg/kg) of Lycium barbarum leaves flavonoids can improve the learning and memory ability of mice exposed to lead; total flavonoids could increase the level of superoxide dismutaselevel (SOD) and cut down the level of malondialdehyde (MDA) in lead poisoned mice brain; Total Flavonoids from Lycium leaf has certain protective lead-exposed mouse dentate gyrus neurons from apoptosis induced by lead.