Lead-induced Learning And Memory Injury And Neuroprotective Effects Of Tanshionne IIA Against Lead-induced Impairment

Lead is a very important neurotoxic substances. Lead exposure can induce adverse effect on multiple organ systems of the human body, particularly impacts on the development nervous system. Tanshinone ⅡA(Tan Ⅱ A) is a kind of quinone extracted from salvia miltiorrhiza, a kind of commonly used Chinese traditional medicine, which has a wide range of pharmacological activity. Studies have shown that apoptosis and oxidative injury mechanism are the main mechanism of lead-induced neurotoxicity. Tan ⅡA has been proved with a great brain protection by inhibiting apoptosis of brain cells and anti-oxidation. Based on the previous study, the program departs from brain protection of lead-induced poisoning. We propose a hypothesis that ’The protective effect of Tanshinone ⅡA on lead-induced nerve damage’, and investigated the underlying neuroprotective and mechanism of Tanshinone ⅡA. The conclusions as follows:Lead injury the learning and memory abilities of rat. We establish the lead poisoning model and protective model with Tanshinone ⅡA, using the Morris water maze behavioral test method to inspect the learning and memory ability of each group, while using atomic absorption spectroscopy to detect blood lead levels(BLLs) of rats. Our study demonstrated that rats in the lead toxicity group had higher blood lead level with decreased learning and memory ability. While Tanshinone ⅡA intervention relieved the lead induced decrease.Neuroprotective effects of Tanshionne ⅡA on lead-induced Impairment. In order to explore the effect of lead exposure on brain cell apoptosis, we have used the Western blot and immunohistochemistry methods to investigate the mechanism of lead-induced cell apoptosis. The result demonstrated that the lead exposure effect the expression of Bax and Bcl-2 on brain tissue, and Tanshinone ⅡA intervention can reverse the situation of lead-induced abnormal expression. Therefore, revealing the apoptotic mechanism of Tan ⅡA protecting lead-induced poisoning in brain in further.Furthermore, we also investigate the oxidative stress damage of lead and the anti-oxidation of Tanshinone ⅡA on the brain. By comparative the oxidative indexes between Tan IIA protection group and lead poisoning group, Lead causes oxidative indexes abnormal in rats and Tan ⅡA can significantly enhance the activity of SOD and GSH levels (p<0.05), reduce the levels of MDA(p<0.05)...