The Implication Of Cell Apoptosis Imbalance And FEN1Gene Mutation In The Pathogenesis Of Lupus Nephritis

Objective:The aim of our study is to estimate the role of cell apoptosis imbalance and FEN1gene mutation in the pathogenesis of lupus nephritis. Methods:(1)30paraffin organizations from renal biopsy of patients with LN was used terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL) to detect the cell apoptosis, the relation of LN pathology classification, SLE activity index2000(SLEDAI2000) and kidney tissue cell apoptosis index (AI) was discussed.(2) We analyzed FENl gene61563142～61563342by extraction of genomic DNA from the peripheral blood of50LN patients,25healthy individuals and20patients with cancer of the stomach and performed genotyping using DNA sequencing.Results:(1) There was diffused apoptosis identified in all samples of30LN patients(LN-Ⅳ40.0%, LN-Ⅱ26.67%, LN-Ⅲ6.67%, LN-V13.33%and LN-Ⅵ3.33%, no slight change disease), correlation analysis showed that SLEDAI2000was significantly correlated with apoptosis index(0<r<1, P<0.05).(2) FEN1gene61563142～61563342was not significant difference between LN patients, healthy controls and patients with stomachcancer.Conclusion:Our date suggested the cell apoptosis dysfunction plays a key role in the pathogenesis of LN. Although FEN1gene61563142～61563342was not found significant difference in LN patients, larger sample or Genome-Wide Association Studies was needed.