| Objective:To observe the effects of Esculemoside A(EsA)on apoptosis and expression of Bax and Bcl-2 in nephridial tissue of BXSB mice. In order to explore the mechanism by which ESA effect the Lupus nephritis. Methods:24 male BXSB mice at the age of 18 weeks were randomly divided into three groups:untreated model group,EsA-treated group and Dexamethasone-treated group. Three groups BXSB mice were treated by Sodium Chloride,ESA and Dexamethasone.4 weeks later,All mice were killed to obtain Urine,serum and nephridial tissue. The following parameter was detected:1. Urine protein and urine creatinine concentration.2.serum creatinine and urea nitrogen concentration.3.Renal Pathology.4.the apoptosis ratio of nephros cells.5.the expression of Bax and Bcl-2 in nephridial tissue.Results:1.At the end of experimentation,the level of urine protein/urine creatinine,serum creatinine and urea nitrogen concentration were decreased in EsA-treated and Dexamethasone-treated group (P<0.05).There is no difference between EsA-treated and Dexamethasone-treated group(P>0.05).2.After drug treatment, the renal histopathologic of BXSB mice moderate significantly.3.The apoptosis index of nephros cells is different among three groups(P<0.05). The apoptosis index of EsA-treated group is highest, Dexamethasone-treated group is following.4.The expression of Bcl-2 in kidneys of untreated group was significantly higher than that of EsA-treated and Dexamethasone-treated group (P<0.05).There is no difference between EsA-treated and Dexamethasone-treated group(P>0.05); As for Bax,it express in three groups,but the difference has no significance(P>0.05);the ratio of Bcl-2/Bax is different among three groups(P<0.05). Conclusions:I.EsA can degrade urine protein concentration and improve renal function,pathological kidney damage in BXSB mice.2.EsA can promote the apoptosis of nephros cell and down regulation the expression of Bcl-2 in BXSB mice. |
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