Study On The Compound Bone Morphogenetic Protein(BMP) With Chitosan And Cyanoacrylate On Preventive Absorption Of Rat’s Alveolar Ridge

Object:1. Using the bone morphogenetic protein-2, cyanoacrylate and chitosan and others to made the compound biological glue. To study the physical, chemical and biological properties of this compound.2. To evaluate the effectiveness and feasibility of implantation of the compound bone morphogenetic protein with P-cyanoacrylate and chitosan to preventive absorption of residual alveolar ridge of rat lower incisor alveolar fossa.Methods:1. Bone morphogenetic protein (BMP), cyanoacrylate and soluble chitosan were be used to make the compound cyanoacrylate-chitosan-BMP. To use the cyanoacrylate, chitosan and glycerophosphate make the chitosan temperature sensitive biological glue.2.54wistar rats were divided randomly and equally into three groups: experimental group (compound cyanoacrylate-chitosan-BMP group), control group (chitosan composite group) and blank control group.3. Animal models, use the needle holder to clamp off the lower incisors (3times,1times/3days) before extraction. After three times, extract the incisors.4. Compound cyanoacrylate-chitosan-BMP was implanted into rats’alveolar fossa of the experimental group, the chitosan temperature sensitive biological glue was implanted into alveolar fossa of the control group, and alveolar fossa of the blank control group was implanted nothing. Animals were sacrificed after3,6,9weeks, and separated and removed all rats’mandible of every group.5. To use the mammography scanner take the X-ray film of rats’mandible, to use the software of Image-Pro Plus6.0measured and analysis the X-ray film. Routine histological observation:The joint specimens were exposed; fixed by neutral formalin, colored by HE staining, wrapped and cut into slices by4μm, study and observed them under a microscope.Results:1. Compound cyanoacrylate-chitosan-BMP is one kind of glue, which is temperature-sensitive, semi-solid, illiquid, and elastic. There was100μg rhBMP-2in one liter.2. The relative length values of residual alveolar ridge and optical density test in experimental group was all greater than those of other groups at3,6,9weeks after operation. There was significant difference between experimental and control groups (p<0.05).3. Optical microscopy showed that new bone formation rate and quality in the experimental group was better than those in the control group and blank control group at3,6,9weeks after implantation.Conclusion:Compound cyanoacrylate-chitosan-BMP has good biocompatibility, safe, stability and ability of osteoinduction. It can be used to prevent the absorption of residual alveolar ridge and have more clinical predications.