| Objective To observe the influence of chemotherapy in different time on wound healing, the VEGF express in tissue at wound and TNF-a level in serum in order to define the optimal time of chemotherapy during operation. Furthermore, observe the effect of G-CSF and Kangfuxinye to wound healing, and explore mechanism for providing theoretical bases.Methods Part1. New Zealand white rabbits (48cases), were randomly divided into6groups:normal group(8cases) was given surgery, other groups(8cases every group) were given infusion of epirubicin respectively on the day before the operation, three days before operation, two days after operation, four days after operation and six days after operation. At the same time to observe the VEGF express, TNF-a level and states of wound at the1st day,3rd day,5th day and7th day.Part2. The rabbit(40cases) were randomly divided into5groups:normal group,control group, G-CSF group and Kangfuxinye high close treated group, Kangfuxinye low dose treat group. After7days of operation combing with chemotherapy, the grade of incision, the status of pathology, the level of VEGF and the level of TNF-a were observed.Results Part1. There was worse effect of chemotherapy to wound healing on the day before the operation (p<0.05), the infected degree is higher than normal group. But there was no significant difference at three day before, two days after or three days after operation. For the VEGF levels, no difference was found in various groups. The TNF-α levels,9.8±4.31pg/mL on the day before the operation group,11.1±3.8pg/mL on two days after operation group,11.3±3.18pg/mL on four days after operation group, were lower than normal group (p<0.05)Part2. The differences were observed including wound healing, VEGF and TNF-a in7th day after surgery. The VEGF in G-CSF group and Kangfuxinye high dose treated group were increased and the wound healing was better than the control group, but there was no difference of TNF-a in them.Conclusion1. The chemotherapy on the day before the operation would cause the greatest worse effect of wound healing.2. No difference of VEGF level were found in various time of operation with chemotherapy, but the TNF-α levels on the day before the operation group, two days after operation, four days after operation group were lower than normal group.3. The G-CSF and Kangfuxinye can increase the VEGF expression of incision sites, and it is helpful to wound healing after treatment with both chemotherapy and surgery. |
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