The Study Of BDNF In Osteogenic Differentiation Of BMSCs

Fracture healing is controlled by many local and systemic regulatory factors.Recently， molecular studies revealed that cellular proliferation, chondrogenesis,osteogenesis, and certain peptide growth factors may play important roles in fracturehealing process. Bone marrow stromal cells (BMSCs) are multipotential stem cells,capable of differentiating into bone, cartilage, fat and muscle. Transplantation of bonemarrow stromal cells (BMSCs) has been regarded as a potential approach forpromoting nerve regeneration. Recently, several reports described bone marrowstromal cells expressed certain neurotrophic factors could repair fracture healing.BMSCs were obtained by Percoll gradient centrifugation technique. BMSCswere induced to neural-like cells by basic fibroblastic growth factor(bFGF) andEpidermal growth factor (EGF). We designed the the eukaryotic expression vectorpECFP-C1-BDNF. Transfected the recombination plasmids into BMSCs andsubjected to Western blotting analysis.In our study, we have succeed to construct the eukaryotic expression vector, andtransfected the pECFP-C1-BDNF vector into mesenchymal stem cells, also producedBDNF proteins in cells. In our investigation we found that BDNF produced byBMSCs have enhanced osteogenic differentiation of BMSC. The finding that BDNFon bone forming cells suggests that it may play an important role in the regulation ofbone formation.