| Objective: Lanthanum compounds have the great prospective on the biomedical applications.But La3+ iron could easily form the precipitate with phosphate group in the cell medium.However,it is unknown that what kind of lanthanum-containing precipitation could form in the medium,and which kind of lanthanum-containing precipitation could affect the biological effect of cells.Because of the bone affinity of lanthanum,this paper aimed to study the species of La?NO3?3 dispersed in the cell medium and the osteoblasts differentiation of the bone marrow stroma cells?BMSCs?treated with different lanthanum-containing precipitation.Methods: 1.Different lanthanum-containing precipitation were obtained by adding various concentrations of La?NO3?3 solutions to the DMEM or DMEM with FBS.A series of characterization methods including DLS,SEM,TEM,EDS and protein quantification were employed to clarify the species of the different lanthanum-containing precipitation.2.The primary BMSCs were isolated and cultured firstly.Then the cell viabilities,ALP activity and the formation of mineralized nodule of BMSCs were tested when treated with different lanthanum-containing precipitation.Results: 1.La?NO3?3 solutions in DMEM culture could form La PO4,which exits in the particle formation.La?NO3?3 solutions in DMEM culture with FBS could form La-PO4-protein compound.2.When treated with La?NO3?3 solutions in DMEM culture,the cell viability of BMSCs was inhibited at the concentrations of 1,10 and 100 ?mol/L at the first day and the third day,meanwhile the supernatent derived from the La?NO3?3 solutions in DMEM culture could not affect the cell viability of BMSCs.And the precipitate derived from the La?NO3?3 solutions in DMEM culture added to the complete medium could inhibit the cell viability of BMSCs at the concentrations of 10 and 100 ?mol/L.When treated with La?NO3?3 solutions in DMEM culture with FBS,the precipitate and supernatent derived from the La?NO3?3 solutions in DMEM culture with FBS,could not affect the cell viability of BMSCs except for the concentration of 100 ?mol/L La?NO3?3.3.The La-PO4-protein formed from the La?NO3?3 solutions in DMEM culture with FBS could inhibit the osteoblast differentiation of BMSCs at the concentration of 1 ?mol/L La?NO3?3?P<0.05?,but had no effect on the osteoblast differentiation at the concentrations of 0.001 and 0.1 ?mol/L La?NO3?3;and had no effect on the formation of mineralized nodule at all tested concentrations of La?NO3?3.The precipitate derived from the La?NO3?3 solutions in DMEM culture added to the complete medium had no effect on the osteoblast differentiation of BMSCs at the concentrations of 0.001-0.1 ?mol/L La?NO3?3,but could inhibit the osteoblast differentiation at the concentrations of 1 ?mol/L La?NO3?3?P<0.05?;and had no effect on the formation of mineralized nodule at all tested concentrations of La?NO3?3.Conclusion: 1.La?NO3?3 solutions in different cell culture medium could form different lanthanum-containing compounds: La PO4 particles?in the DMEM medium?and La-PO4-protein compound?in the DMEM medium with FBS?.2.The different lanthanum-containing compounds will cause the different cell viability of BMSCs.3.The different lanthanum-containing compounds will cause the different effects on the osteoblast differentiation and the formation of mineralized nodule of BMSCs.4.The lanthanum-containing precipitation inhibit the osteoblast differentiation by inhibit the expression of osteoblast genes and proteins. |
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