The Mechanism Of Basic Fibroblast Growth Factor In Mediating Increase Of Intracellular Free Magnesium In Vessel Endothelial Cells

Objective:The mechanism of basic fibroblast growth factor in mediating increase of intracellular free magnesium in human umbilical vein endothelial cellsMethods:By collagenase digestion, fresh human umbilical cords were washed and centrifugated to gained HUVECs. HUVECs were maintained in M199medium supplemented with20%fetal bovine serum and placed in24hole cultivated board at37℃in a5%CO2incubator. The primary cultured cells used in this study were between passages2and4passages. Marked with the fluorescence indicator mag-fura-2-AM and By the Whole cell patch clamp technique, using PTi ion fluorescence method to detect [Mg2+]i in HUVECs dynamically.Results:At resting state, The mean basal level of [Mg2+]i in the HUVECs was (0.52±0.02) mmol·L-1。In the presence of extracellular Mg2+0mmol·L-1、1mmol·L-1and2mmol·L-1, The level of [Mg2+]i increase in HUVECs at (0.78±0.13) mmol·L-1、(0.76±0.11)mmol L-1and (0.74±0.14) mmol·L-1in order.bFGF also increased [Mg2+]i in a dose-depen-dent manner with an EC50of (12.03±1.08) ng/ml. In the presence of extracellular BAPTA-AM100mmol·L-1、Ommol·L-1, The level of [Mg2+]i increase in HUVECs at (0.78±0.14) mmol·L-1and (0.76±0.11) mmol·L-1. In the presence of extracellular Na+145mmol·L-10mmol·L-1, The level of [Mg2+]i increase in HUVECs at (0.76±0.12) mmol·L-1and (0.75±0.12) mmol·L-1. HUVECs pretreatmented with goat IgG and IPK3B-Ab, the bFGF-induced increase of [Mg2+]i was (0.76±0.17) mmol·L-1and (0.18±0.15) mmol·L-1.Conclusion:bFGF induced the release of Mg2+from intracellular Mg2+stores through the IP3kinase signaling pathways in HUVECs.