| OBJECTIVE: To study the influence of basic fibroblast growth factor (bFGF) by local application methed (soak) on the healing of remove subcutaneous vascular net free skin graft in rats .METHEDS: 10 SD rats, ordinary experiment animals, female and male, body weight 275g ~ 285g, random divided two bundles. Two skin grafts of subcutaneous vascular net were cut off in the back respectively and then transplanted in situ. The upside skin grafts used as test group, free skin graft soak 10 minuts in bFGF, and downside skin grafts were used as control group, free skin graft soak 10 minuts in NaCl. After operation , the survival of skin grafts were generally observed by naked eye. The skin grafts were sampled to observe numbers of blood capillary and growth of granulation tissues with microscope.RESULTS : The survival of skin grafts in the test and control groups were 95% and 72% respectively (t=1.753 , p<0.01) .Under the microscope, the surviving area of experiment bundle have a great deal granulation tissues, a large number newly born blood vessels, distinctly below muscular layer. There were significant differences in the numbers of blood capillary and growth of granulation tissues between the two groups (p<0.05). There were significant differences in growth of granulation tissues (p<0.01).CONCLUSION : bFGF, can directly accelerate proliferate and differentiation of fibroblasts, blood vessal endothelial cells, smooth muscle cells in granulation tissue, stimulate the formation of granulation tissue, promote blood capillary proliferation, increase the number of blood vessal in granulation tissue and removed free skin graft, accretion the diameter of blood vessal, so improve growth celaration of granulation tissue , promote the surviving of removed free skin graft. bFGF, as an important vascularization factor, has distinctly auxo-action to the surviving of removed free skin graft. bFGF can increase proliferate of epiderric cell, succeed the healing process between removed free... |
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