The Effects Of Borneol’s Role Of Leading Drug Distributing Upwards On Acute Alcoholism Mice

Objectives:To investigate the effects of borneol’s role of leading drug distributing upwards on acute alcoholism mice and to explore the possible mechanism.To provide drug reference for Chinese medicine in treating acute alcoholism.Methods:SPF mice. male.20g±2g. were randomly divided into normal group, control group A. control group B and experimental group. The normal group was fed with normal saline by gavage while the control group A was fed with56%alc/vol, the control group B was fed with suspension mixed with saline and borneol, the experimental group was fed with56%alc/vol alchol solution containing borneol concentration of14mg/ml. Each group was fed with normal diet fora week after normal environmental adaptability feeding then every group was given one-time intragastric administration (gavage volume is14ml/kg).All groups were fasted but water for12hours before the experiment.To observe the ebriety time and sober time so as to calculate ebriety rate and death rate.After the administration, there were seven time-points namely10min,30min,1h,1.5h,2h,3h,6h at which we would test blood alcohol concentration.To examine ALT. AST and ALP in serum and SOD&MDA in brain tissue after six hours and make liver pathological examination.To test content of DA and5-HT in brain striatum one hour later while doing immunohistoch-emical determination of striatal DA and5-HT by calculating IOD of immunoreactive products after one hour and six hours.Results:1. The ebriety time of experimental group was shortened while the sober time was prolonged apparently. Compared with control group B, the difference was statistically significant (P<0.05); the death rate of experimental group was the highest and control group A was higher, compared with the other groups, the differences were statistically significant (P<0.05).2. The peak of blood alcohol concentration of experimental group was higher than control group A and it took less time to get it while dropped faster too. Compared with control group A, the differences of others time-points were statistically significant (P<0.05) except the time point of10min.3. The content of AST, ALT and ALP in serum and MDA in brain was increased while the activity of SOD was decreased in experimental group and so as to control groupA too. However, the former was more apparent than the latter. Compared with the other groups, the differences were statistically significant (P<0.05).On the other hand, control group B did not change too much,the difference was not statistically significant (P>0.05) when compared with normal group.4. The content of DA in experimental group was increased obviously, and control groupA too. But the former was more apparent than the latter. Compared with the other groups, the differences were statistically significant (P<0.05).The content of5-HT in all groups were increased but nomal group, and the differences were statistically significant (P<0.05) when compared with it while the experimental group was the most obvious one. After both one hour and six hours. IOD of immunoreactive products of DA and5-HT in all groups were increased but nomal group, and the differences were statistically significant (P<0.05) when compared with it while the experimental group was the most obvious one.However, the condition after one hour was much more obvious than six hours later, and the differences were statistically significant (P<0.05).Conclusions:1. Borneol has an effect of shortening ebriety time and prolonging sober time apparently while increasing death rate on acute alcoholism mice.2. Borneol has an effect of accelerating absorption and metabolism of alcohol on acute alcoholism mice.3. Borneol has an effect of increasing brain injuries on acute alcoholism mice. 4. Borneol has an effect of increasing neurotransmitters disorder in brain on acute alcoholism mice.The disorser one hour later is much more apparently than six hours after intragastric administration.