Separation And Identification Of EV71in Changchun Area And The Antiviral Pharmacodynamics Research Of Shuqing Keli

Enterovirus type71（Enterovirus71, EV71） belonging to the small RNA virusunit, which is a single-strand RNA virus of enterovirus genus. Due to its pathogenicityand the high death rate, the hand-foot-mouth disease becomes a serious threat toinfants ’ and children’s health in recent years, the fatality rate of its severe cases is inrising trend, and it was already been put on a list of Group C management ofinfectious diseases by ministry of health. At present, the EV71vaccine is still inexperimental stage, there is no mature product used in prevention, and lack ofeffective treatments, it put a serious impact on public health in the world, therefore,the development of effective drugs to combat EV71infections is of great significance.As a treasure of the Chinese nation, Chinese traditional medicine accumulatedrich experience to the prevention and treatment of diseases caused by viruses forthousands of years. A substantial amount of literature indicates that Chinesetraditional medicine has the integrated functions of: to inhibit the replication of thevirus, to prevent the cytopathic caused by virus infection, analgesic andanti-inflammatory, and to regulate immune functions, which has broad applicationprospects.Shu Qing granules as new antiviral drug, provides new prescriptions oftraditional Chinese medicine on clinical treatment of EV71infection. In this study, wecollected5suspected cases of Ev71specimens, after the isolation by RD cell culture,all specimens appeared marked cytopathic effects. The cellular morphology becomesmore rounded, smaller, arranged more scattered, cytoplasmic granules increased andits refraction enhanced. The cells drop off from the culture flask and floating in theculture fluid.After RT-PCR validation, a strain-specific striping occurs, the positive rate is20%. Determination of the EV71virus titers is106.32TCID50/ml. The inhibit50%cell growth drug concentration （CC50） of Shu Qing granules and granules of radixisatidisare are:50±0.39mg.ml^-1，15.67±0.32mg.ml^-1. The half effectiveconcentration （IC50） on inhibition of EV71proliferation are （0.136±0.022） and （0.199±0.016） g· L^-1, respectively. Research results showed that compared withgranules of radix isatidisare, the impact of Shu Qing granules on cell proliferation issmaller, and its antiviral effect is clearer. Further experiments showed that the drugscan significantly inhibit the process of virus adsorption. Compared with the granulesof radix isatidisare group, Shu Qing granules can significantly inhibits theproliferation of EV71in vitro, which provides a new method for clinical treatment ofhand-foot-mouth disease caused by EV71infection.