Expression Changes Of COX-2and Lymphatic Vessel Density In Oral Squamous Cell Carcinoma Before And After Chemotherapy

Object:To detect the expression of COX-2in the Oral squamous cell carcinoma and the change of lymphatic vessel density(LVD) between inside and around the carcinoma in before and after intravenous chemotherapy and interstitial chemotherapy. Furthermore, to discuss the influence of the preoperative chemotherapy in lymphatic generation of Oral squamous cell carcinoma, lymphatic vessel density and lymphatic metastasis.Clinic Data and Method:To choose60Oral squamous cell carcinoma which are embedding block samples as study object from postoperative patients. Hereinto,30pieces were simple surgery patients,15pieces were preoperative pingyangmycin with intravenous chemotherapy combined surgery,15pieces were preoperative patients who were taken in interstitial chemotherapy combined surgery with5-FU slow release formulation. Here we adopt immunohistochemical method to detect the expression of COX-2and lymphatic vessel positive expression rate that is marked with D2-40in Oral squamous cell carcinoma tissue. Then, compute the lymphatic vessel density from inside and around the carcinoma separately.Result:In the control group of Oral squamous cell carcinoma, the positive expression rate of COX-2from the group which is without chemotherapy is83.3%, while the intravenous chemotherapy and interstitial chemotherapy groups are66.6%and40%. To compare the positive expression rate between the first group who were without chemotherapy and the other two groups, the difference is statistically significant (P<0.05), and to compare the intravenous chemotherapy group with the interstitial chemotherapy group, the difference is statistically significant (P<0.05). COX-2expression and lymph node metastasis were positively correlated. To compare the round of carcinoma with the inside of it in the lymphatic vessel positive expression that is marked with D2-40group, the difference is statistically significant (P<0.05). The LVD is2.25±1.28and9.14±3.82in tumor tissue and around the carcinoma, to compare the two groups, it is significant difference. It is12.22±2.25that the average LVD around the carcinoma in the group that without chemotherapy, while for intravenous chemotherapy and interstitial chemotherapy are8.12±0.79and4.02±0.88. The difference is statistically significant (P<0.05) between the three of them. To compare the intravenous chemotherapy LVD around the carcinoma with interstitial chemotherapy group, the difference is statistically significant (P<0.05). To compare LVD between the lymph node metastasis group and one without lymph node metastasis, the difference is statistically significant (P<0.05). In addition, to compare LVD between COX-2expression positive group with COX-2expression negative group, the difference is statistically significant (P<0.05). Comparing with the average LVD of three groups in tumor tissue, the difference is not statistically significant (P>0.05). To compare the LVD with COX-2expression between positive patients and negative patients who were and won’t accepted chemotherapy before surgery, the difference is statistically significant (P<0.05). The COX-2expression and the LVD around carcinoma in patients were without chamotherapy before surgery and Lymph node metastasis were positively correlated. The COX-2expression and the LVD around carcinoma in patients were with chemotherapy before surgery and lymph node metastasis don’t have significant relationship.Conclusion:When reducing the COX-2expression rate and the LVD which around the carcinoma with preoperative intravenous chemotherapy and interstitial chemotherapy, the latter is more remarkable. Reducing the COX-2expression rate and restraining the lymphatic vessel formation around the carcinoma by preoperative chemotherapy to decrease lymph node metastasis.