Effect Of Autologous Bone Marrow Mesenchymal Stem Cell Transplantation On Motor Function In Children With Cerebral Palsy

ObjectiveUsing GMFM88and FMFM45scale to evaluate the effect of autologousbone marrow mesenchymal stem cells (BM-MSCs) transplantation on motorfunction in children with cerebral palsy.Methods20cases of cerebral palsy children were hospitalized in the GeneralHospital of Chinese People’s Armed Police Forces from December2010to July2011. After excluded from family history, genetic diseases, infectious diseases,congenital diseases,45ml autologous bone marrow were extracted from eachchild and were mixed with2ml heparin, then were immediately sent to sterile cellroom. Autologous bone marrow derived mesenchymal stem cells were culturedunder strict sterile conditions. GMFM88and FMFM45scale were used toevaluate the effect of autologous bone marrow mesenchymal stem cells on themotor function of these patients before and1,3and6months after treatment.Self-control was used to evaluate the motor function before and after thetransplantation. Values of GMFM and FMFM before and1,3and6months afterthe transplantation were statistically analyzed to evaluate the therapeutic effect.RESULTS17subjects successfully completed the clinical study, the results ofstatistical analysis by SPSS13.0software showed that:1. The A, B and C functional area scores and total score of GMFM88significantly increased at the end of1,3and6months after BM-MSCstransplantation than before (P <0.05). The improvement of the lying and turning over function in A area, the sitting function in B area, the crawling and kneelingfunction in C area were the most significant. The values in A, B and C area werecontinuously improved even3and6months after treatment. Though the valueof D and E areas also increased3and6months after treatment, but there wasnot statically significant.2. All the values of the upper extremities movement in B area and of thegrasping in C area, initial gross score and gross score improved significant1,3and6months after treatment assayed with FMFM45(P <0.05). Neither obviousside effects or adverse events occurred during the treatment and the6monthsfollow up.CONCLUSIONSBM-MSCs transplantation is a safe method to effectively improve the motorfunction of cerebral palsy patients. The gross and fine motor function was mostrapidly improved in1month after the therapy and it could be continuouslyimproved within6months after the therapy.