Analysis Of6257Cases Of Cervical Cytology On Genotyping For HPV And The Value Of The HPV Detection In Following-up Of CIN After LEEP

objective1.To investigate the distribution of the human papillomavirus subtypesinfection of different age groups of female, to understand the distributioncharacteristics of HPV subtype infection.2.To investigate HPV subtypes’s distribution in different cervical lesions, toelucidate the clinical value of early diagnosis and treatment of cervical lesions.3.To investigate the significance of HPV subtypes of cervical intraepithelialneoplasia after loop electrosurgical excision procedure and the prognosis of CIN.Methods1.Choice of cases:6257cases selected from the gynecologic department ofthe hospital were tested HPV genotypes between January2009to2011March,aged20to76years old, mean age38.5±11.5years old.According to theage, all cases are divided into4groups (20to29years old,30-39years old,40-49years old,≥50years old).2.Experimental methods:6257patients underwent TCT and HPV genotyping(21genotypes) test using flow-through hybridization gene chip technology.1280cases whose results of TCT were abnormal.These cases underwent cervicalpathology examination.304cases’ pathologic examination results were CIN andwere treated with the Leep types in the hospital.6months,1to2years after LEEP,all cases underwent TCT and HPV. If the result of tests were abnormal, we wouldunderwent cervical pathology examination again. Results1.The relationship between HPV subtype infection and ageThe HPV infection rates of different age groups were different (p<0.05),HR-HPV infection rates of different age groups were different (P<0.05). TheLR-HPV infection rates of each age group were different (P>0.05). Each agegroup HPV mixed-infection rates were different (P<0.05).The top five of hpvsubtypes were16,52,11,33,31.The infection rate of HPV16in all age groupsare located in the first.2. The different subtypes of HPV infection in cervical lesionsHPV infection rates of different level cervical lesions weredifferent(P<0.05),and the infection rate of HR-HPV were different(P<0.05). HPVinfection rate, HR-HPV infection rate increased with the degree of cervical lesionsgradually increased.HPV multiple infection rates were different, but notsignificance, the single infection rates were different (P<0.05).3. The value of HPV testing in following-up of CIN postoperative(1)Before LEEP,6months and1to2years after LEEP,304cases’ HR-HPVinfection rates were different (P<0.05).6months after LEEP, patients with CIN I,CIN II, CIN III HR-HPV infection rates respectively were16.8%,21.9%,28.0%,the difference was not statistically significant (P>0.05).1to2years after LEEP,HR-HPV infection rates of CIN I, CIN II, CIN III respectively were3.0%,5.2%,8.4%, the difference was not statistically significant (P>0.05).(2)With CIN levels increasing, HR-HPV clearance rate were not significantlydifferent6months after LEEP. With CIN levels increasing, HR-HPV clearance ratewere not significantly different1to2years after LEEP.6months,1to2yearsafter LEEP, the clearance rate of HR-HPV respectively were different (P<0.05).1to2years,HR-HPV-positive group’s recurrence rate was higher thanHPV-negative group and LR-HPV-positive group.Conclusions1.HPV infection rates were different among different age group. Thedistribution of HPV subtypes were different among different age groups. High-riskpopulation should be tested HR-HPV.2.With the increased levels of cervical lesions, the trend of HPV infectionrates are increasing, the trend of HR-HPV infection rates are increasing. The HR-HPV infection is a high-risk factor for cervical cancer.3.LEEP can effectively remove HR-HPV infection. As time goes by, clearancerate of HR-HPV gradually increased. The recurrence of HR-HPV infection groupwas higher than other groups.HR-HPV persistent infection is the importantwarning of CIN recrudescence after LEEP.