Curcumin Attenuates High-glucose-concentration-induced RF/6A Damage

ObjectiveTo explore protective effects of curcumin on high glucose concentrationdamaged rhesus choroido-retinal endothelial cell (RF/6A) in vitro.MethodsRF/6A was assinged into4groups (n=6/group). Control group (CONTgroup): DMEM+10%FBS, high glucose concentration group (HGC group):DMEM+10%FBS+30mmol/L glucose, curcumin group (CUR group):DMEM+10%FBS+30μmol/L curcumin, curcumin-glucose group (C-G group):DMEM+10%FBS+30mmol/L glucose+30μmol/L curcumin. Cell viability wasdetected by methyl thiazolyl tetrazolium (MTT) at1,3and5d. Moreover,malonaldehyde (MDA) and activity of superoxide dismutase (SOD) weredetected, besides, agarose gel electrophoresis was introduce to investigateDNA ladder in each group. We also detected expression of proliferating cellnuclear antigen (PCNA) and anti-oncogene protein27(P27).ResultsCompared with CONT group, cell viability in HGC group was decreasedin time dependence manner (P<0.01), and showed no obvious difference inCUR and C-G groups (P>0.05). Detection of oxidative stress revealed that5dafter RF/6A cultered, MDA in HGC group was significantly increased (P<0.01),while, activity of SOD was decrease compared with CONT group (P<0.01),However, no difference could be detected in CUR group and C-G group,compared with CONT group (P>0.05). Agarose gel electrophoresis showed that HGC group presenting with obvious DNA ladder, while no ladder wasobserved in CONT CUR group or C-G group. Furthermore, compared withCONT group, PCNA was down-regulated and P27was up-regulated in HGCgroup (P<0.01), and no difference was presented either in CUR group or inC-G group (P>0.05).Conclusions1. High glucose concentration (HGC) induces oxidative stress in RF/6A,down-regulating PCNA while up-regulating P27, inducing apoptosis, andtherefore decreasing cell viability2. Curcumin inhibits HGC-induced oxidative stress in RF/6A, reversingexpression of PCNA and P27, inhibiting apoptosis, and thus normalizingcell viability.3. Curcumin has no effect of on oxidative, expression of PCNA and P27,apoptosis or cell viability under normal condition.