A Study On Expression Of HMGB1 In Vascular Endothelial Cells And The Effect On Oxidative Stress By High Glucose

Objective: To observe the effects of high glucose on the expression of HMGB1 in cultured vascular endothelial cells (VECs) and the effect of the culture fluid with high glucose condition on oxidative stress.Methods 1. The human umbilical veins endothelial line (Ea.hy926) were cultured in vitro. VECs were cultured in medium with different glucose concentrations for 48h. The glucose concentrations were 5.6 mmol/L (normal control), 20 mmol/L (high glucose group 1), and 33mmol/L (high glucose group 2) .The levels of HMGB1 in the supernatant were measured by Western blot. The cell viability within 48h was examined by trypan blue exclusion test. 2. VECs were divided into two groups: high glucose group (33mmol/L) and the mixed group (the supernatant of high glucose group 2 plus 33mmol/L high glucose medium). 24 hours later, the levels of MDA and SOD were measured by UV-Spectrometer.Results 1. Compared with normal control, the expression of HMGB1 was significantly increased in the supernatant of high glucose group(P<0.05). Within 48h, cell viability of all groups were over 95%, and no differences in cell viability were found compared with control group by Trypan blue exclusion test. 2. The level of MDA was significantly higher in the mixed group then that in 33mmo/L high glucose group. And the activity of SOD was lower in the mixed group than that in high glucose group (p<0.05). Conclusion High glucose could significantly induce the expression of HMGB1 in cultured VESs. In high glucose condition, the level of HMGB1 increase, which may play a role in oxidative stress and promote the injury of vascular endothelial cells.