TIn Vitro Study Of Cisplatin And Ciprofloxacin And Combination Inhibiting Human Hepatoma Cell Bel-7402

Objective To observe changes of proliferation and healing and migration treated Cisplatin(DDP),one of the most common antitumor drugs used in clinic,or Ciprofloxacin(CIP),one of Quinolone antibacterial drugs used in clinic,were chosed to investigate their cytotoxic effects on human hepatoma cell line of BEL-7402in vitro.And the effect of their colalition on the key gene of migration, MMPs and possible molecular mechanism were evaluated to settle foundation for using those to cure liver-cancer in clinic.Methods BEL-7402cells were cultured in vitro and divided into four groups:control, Cisplatin-treated,Ciprofloxacin-treated and combination group. Exponentially growing cells were chosen for experiment.The growth inhibition rate and IC50value were calculated based on MTT assay.Cellular healing and migration ability were determined by cell scratch wound and migration assey. inhibitive ratio in each assay was calculated. The changes of mmp2and mmp9coresponding to cancer metastasis were evaluated by reverse transcription polymerase chain reaction(RT-PCR) after treatment of the drugs.Results1.Individual DDP and CIP and combination all could restrain the growth and healing and migration of BEL-7402cells and in dose-dependent manner(P<0.05).Compared with DDP and CIP,the combination showed more superiority(P<0.01),except CIP50μmol/L.2.The DDP IC50was11.2±0.6μmol/L,and the CIP was202.3±4.3μmol/L,and combination9+60～12+250μmol/L.3.RT-RCR data showed that the transcription level of MMP-2and MMP-9in cells was significantly inhibited by DDP and CIP as well as and combination(P<0.01),and the combination was more obvious advantage.Conclusions1.DDP and CIP alone or combination inhibit the proliferation,healing and migration ability of human hepatoma cell BEL-7402in a dose-dependent manner;2.Coadministration of DDP with CIP showed additive inhibitory effect;3.DDP and CIP affect invasion and migration ability of human hepatoma cell BEL-7402through lowering expression of MMP2, MMP9genes.