The Vitro Study Of Ascorbic Acid And Cisplatin And Combination Inhibiting Human Hepatoma Cell HepG2

BackgroundPrimary carcinoma of liver is the occurrence of malignant tumors of the liver cells or intrahepatic bile duct epithelial cells. The disease with high incidence and is one of the most common cancers in China. Chemotherapy of hepatocellular carcinoma is one of the therapeutic method, but the traditional antitumor drug have the side effects of such as bone marrow toxicity, liver and kidney toxicity, and produce drug resistance. Herefore searching drugs with low toxicity, high effect and low-dose is imperative. In recent years, the domestic and international study demonstrated that vitamin C can inhibits tumor cell proliferation. At present vitamin C plus Cisplatin on hepatocellular carcinoma cell literature rarely reported and we will explore these aspects.ObjectivesTo observe changes of proliferation and healing and migration used Vitamin C, Cisplatin and combination on the human hepatoma cell line HepG2in vitro, and to explore the possible molecular mechanism at the molecular level to settle foundation for using those to cure liver cancer in clinic.MethodsHepG2cells were cultured in vitro and divided into four groups:control, Vitamin C-treated, Cisplatin-treated, and combination group. Exponentially growing cells were chosen for experiment. Inverted microscope was employed to observe morphologic changes, the growth inhibition rate and IC50value were calculated based on MTT assay. Cellular healing and migration ability were determined by cell scratch test, the transitional healing rate was calculated. The changes of p53、caspase-3and caspase-8transcription were detected by reverse teanscription polymerase chain reaction(RT-PCR) after Vitamin C in combination with Cisplatin on HepG2cells.Results1Vitamin C, Cisplatin and combination all could restrain the growth of HepG2cells and inhibition effect was enhanced with the increase of drug concentration, had statistical significance compared with that of control group(P<0.05);200μmol/L of Vitamin C in combination with12μmol/L Cisplatin showed synergistic effects of drugs, the concentration of combined group showed additive effects of drugs.2Vitamin C, Cisplatin and combination all could restrain the healing and migration of HepG2cells and inhibition effect was enhanced with the increase of drug concentration, had statistical significance compared with that of control group(P<0.05);3RT-PCR data showed that enhanced expression level of p53, caspase-3and caspase-8of Vitamin C combined with cisplatin on human hepatocellular carcinoma cell line HepG2, had statistical significance compared with that of control group (P<0.05).Conclusions1Vitamin C, Cisplatin and combination all could restrain the growth of HepG2cells and inhibition effect was enhanced with the increase of drug concentration. Combined useing of drugs exhibit synergistic or additive effect.2Vitamin C, Cisplatin and combination all could restrain the healing and migration of HepG2cells.3Vitamin C in combination with Cisplatin inhibit the proliferation of human hepatoma cell line HepG2cells via upregulation the expression of p53、caspase-3and caspase-8.