| Objective: To detect the expression of CD133and CXCR4in colorectal cancer(CRC), and the association between CD133and CXCR4coexpression andclinicopathological factors in CRC patients.Methods: The expression of CXCR4and CD133was evaluated by immunohisto-chemical staining. Associations between immunohistochemical scores andclinicopathologic factors of tissue specimens were evaluated by Pearson Chi’s squaretest. Furthermore,transwell invasion assays were done with HT29and Lovo cancercells, the expression of CXCR4and CD133in cells before and after transwellinvasion assays were compared by immunofuorescence staining.Results: In our study, the expression of CD133and CXCR4was53.3%(24/45) and57.8%(26/45) respectively.46.7%(21/45) tumors were found to overexpression bothCD133and CXCR4. the concomitant high expression of CXCR4and CD133hadstrongly association with the lymph nodal status and distant metastasis and UICCstage (P<0.05). In HT29and Lovo cells, CXCR4and CD133expression were significantly increased after transwell invasion assays as confirmed byimmunofuorescence staining.Conclusions: It is suggested that high expression of both CXCR4and CD133isassociated with advanced colorectal cancers and CXCR4/CD133may play animportant role in CRC cancer cells invasion. |
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