Study On The Correlationship Between Mononuclear Cells’Information And Heat Syndrome In Acute Leukemia

Objective:To explore the association between the mononuclear cells’information of acute leukemia and heat syndrome. It can be the supplement to the macro TCM syndrome information of AL, and contribute to the TCM diagnosis and treatment of AL.Methods:Adopting syndrome element differentiation, the patients were divided into heat and non-heat groups according to the value of heat syndrome. The mononuclear cells(MNCs) were isolated from the patients’marrow and applied for the following analysis. MNCs’ proliferation was measured by MTT assay; differentiation of MNCs was analyzed by NBT assay; adhesion of MNCs to HUVECs was observed through cell number count using phase-contrast microscopy; migration was tested by Millicell. The effect of serving temperature was proliferation of three types of cell lines including:K562, HL-60and U937which were holded in4℃,37℃or45℃for four hours was analyzed by MTT assay to explore the serving temperature of the patients’marrow. The measurement data was collected and analyzed using the software Spss16.0.Results:Among12newly diagnosed AL patients,6cases with the value of heat syndrome>=100were classified as heat group, the others with the value of heat syndrome<70were classified as non-heat group.1. MNCs’ proliferation:at Oh and24h, there was no statistical difference in MNCs’ proliferation was observed between the two group(P>0.05); at48h, MNCs’ proliferation of heat group was lower than that of no heat group(P<0.05). MNCs’ proliferation is going up during0to24h in both groups, however different trends were observed for these two groups in the following24hours. From24h to48h, MNCs’proliferation of heat group switched to decline in heat group, in contrast, it still kept rising in the non-heat group.2. MNCs’ differentiation:there was no statistical difference in MNCs’proliferation between the two groups.3. MNCs’ adhesion:the minimum and maximum value of adhesion was0.207and0.671, but there was no statistical difference in adhesion of MNCs to HUVECs between the two groups.4. MNCs’ migration:the minimum and maximum value of migration was71.9and231.1, but there was no statistical difference in MNCs’migration between the two groups.5. Marrows’ serving temperature:proliferation of all3kinds of cells were inhibited after keeping45℃for4hours; and no statistical difference were found for the proliferation of cells holded in4℃and37℃compared with the control cells which grow in the standard culture condition of37℃and5%CO2.Conclusion:1. The pattern of MNCs proliferation shows an association with the heat syndrome in AL; but MNCs’ differentiation may not correlate with heat syndrome.2. The personal difference of MNCs’ adhesion and migration was observed in AL patients but may be no associated with heat syndrome.3. In this study, the preservation condition of marrows in4℃less than4hours would not affect the proliferation of cells significantly.