The Anti-tumor Effect Of "Shiru Decoction"in Breast Cancer Mouse Model And Its Impact On E-cadherin

Objective:To establish the high spontaneous metastasis breast cancer mouse model. To observethe effects on local and lung metastatic tumors of “Shiru Decoction” and its impact onE-cadherin in Tumor tissue in breast cancer mouse model.Material and methods:We selected randomly40from50syngeneic BALB/c mice to establish the breastcancer model. Using the method of cell suspension injection[1]，4T1cells were injected intosubcutaneous of right upper inguinal region of40BALB/c mice. The7th day，the aimedmodel was established. Then we divided randomly them into four groups: A，B，C，D andgiven therapy as follows: Group A，named as untreated group，0.5ml normal sodium(NS)subcutaneous injection around tumors; Group B0.1ml(20mg/kg)[2]CTX intraperitonealinjection; Group C Give " Shiru Decoction "0.5ml gavage once a day; Group D0.1ml(20mg/kg) CTX intraperitoneal injection and " Shiru Decoction "0.5ml gavageonce a day. At the same time，the rest of eight healthy BALB/c mice were not giventreatment，named as the normal control Group E. All the mice were raised under the samesituation. We observed the growth of tumors every day until the18th day. We selectedrandomly four mice from every group. Harvested tumors,lungs and spleens after theydied， weighted tumors and calculated spleen index. We observed the metastatic situationin lungs with immune-histochemistry method. We monitored the survival time of theremains tumor-bearing groups until they died.All data was analyzed by SPSS17.0for windows.Results:1、After injected4T1cells suspension，40BALB/c mice achieved tumors on the7thday. The tumor formation rate is100%. We randomly divided into four groups，the averageof tumor volumes in four groups are not significantly statistical different(P=0.999).2、After different treatment, the tumor-bearing mice tumor average volume of “Shiru Decoction “group than cyclophosphamide+“Shiru Decoction “group (P <0.05),while less than untreated group (P <0.01), and cyclophosphamide group was notstatistically different (P>0.05). The tumor-bearing mice average tumor weight “ShiruDecoction “group tumor weight was significantly higher than that of cyclophosphamide+“Shiru Decoction “group (P <0.01), while lower than untreated group (P <0.01), andcyclophosphamide group there were no significant difference (P>0.05).3、Each group of breast cancer in mice lung metastasis of cyclophosphamide+“Shiru Decoction “group was obviously lower than lung metastasis in untreated group (P<0.05)). And “Shiru Decoction “group and other groups, lung metastasis rates did notdiffer statistically (P>0.05).4、Each group of mice with breast cancer a median survival of “Shiru Decoction“leader in untreated group (P<0.05), at the same time shorter than cyclophosphamide+“Shiru Decoction “group (P <0.01), and cyclophosphamide compared no significantdifference (P>0.05).5、The tumor-bearing mice spleen index average “Shiru Decoction “group than inthe untreated group and cyclophosphamide group (P <0.05), lower than CTX+“ShiruDecoction “group (P﹤0.01).6、E-cadherin expression after Pearson correlation analysis, the proportion of breastcancer and the survival of tumor-bearing mice was positively related to.Conclusions:1." Shiru Decoction " could depress the growth of tumour，Enhanced E-cadherinexpression,prolong the survival time in breast cancer mouse by gavage. Especially，combined with the low-dose CTX，the efficacy is better.2.4T1established high spontaneous metastasis breast cancer mouse modelE-cadherin expression decreased, and considered as a cancer in mice Positively related tothe prognosis of breast cancer mice." Shiru Decoction "can depress of the E-cadherinlow expression.