The Experiment Study Of Vitamin E’ Antagonism Action To Rats’ Pulmonary Injury Induced By Surrounding Fine Particulate (PM2.5)

Objective: To study the lung injury effect induced by fine particulates of different dose,with the premise of injury effect, and to disscuss the Vitamin E’s antagonist role to theinjury effect induced by PM2.5of different dose. Methods: Firstly, collected fineparticulates and detected the metal element content. Than instructed the animal model oflung toxicity experiment induced by fine particulate applying intratracheal instilled.Fourty-eight healthy, adult and male Wistar rats were randomly divided into eight groupsincluding blank control group, saline control group, three level PM2.5group and threeintervening groups. Saline control group were inoculated saline. Three level PM2.5groupsand three intervening groups were both inoculated with suspensions of PM2.5via trachea1time/week,1.5mg/kg、7.5mg/kg and37.5mg/kg concentration particulate respectively.Three intervening groups were given diet supplemented Vitamin E for fourweeks(500mg/kg,in food). All rats were sacrificed after exposed four times.Bronchoalveolar lavage fluid (BALF) were later examined, including the level of LDH、ACP、AKP and MDA. Blood serum were later examined, including the level of LDH、ACP、AKP、MDA、GSH and SOD. Result: The content of metal element in PM2.5fromhighest to lowest as Fe、Mn、 Pb、Cu、Cr、Cd. The level of ACP、AKP、LDH、MDAin BALF and blood serum have no significantly difference among the saline control groupand blank control group(P＞0.05). The level of each exposed group and each intervenegroup changed markedly comparing with the saline control group(P＞0.05). Each indexamong different dose exposed group increased significantly(P＞0.05), and appeareddose-response relationship, that is to say, the level of all indexes increased with theinstilling dose increasing. Almost all index level decreased markedly among each vitamin E intervene group and the same exposed group(P＜0.05). And the level of four indexesamong each intervene group showed significantly difference(P＜0.05). The activity ofGSH and SOD have no significant among the saline control group and the blank controlgroup(P＞0.05); each exposed groups and each intervene groups have significantlydifference comparing with the saline control group(P＜0.05), and the difference amongeach exposed groups and each intervene groups have been significant(P＜0.05). Theactivity of GSH increased significantly in each intervene groups comparing with the sameexposed group(P＜0.05). The activity of SOD showed significantly difference among theintervene high-dose exposed group and the high-dose exposed group(P＜0.05).Conclusion: Among the metal element species in PM2.5, the highest content was Fe inearthcrust elements and Pb in pollution elements. This study showed that different dosesof PM2.5could resulted in different degree damage effect to rat lung tissue. Vitamin Ecould produced antagonist action to this injury induced by different dose fine particulates.