The Extraction And Assay Of Extracts From Compound Recipe"Qianyu" Which Is Used In The Therapy Of Chronic Prostatitis

ObjectiveTo improve the way to administer centipede. Comparing protein content and protein gel bands characteristic changes between boiling and ultrasonic in different time. Comparing protein content and protein gel changes between boiling six kinds of herbs together and boiling the other five herbs first, then centipede. To extract and assay polysaccharides, flavonoids, Saponins, alkaloid which providing the abstraction technology for the animal potency experiments and in vivo action researchMethodsCoomassie blue-UV spectrophotometric determination is used in protein content of the extracting solution; total protein electrophoresis gel of extracting solution is obtained by polyacrylamide gel electrophoresis technology; the bands of protein content and electrophoresis gel is analyzed by t-test.To extract different chemical parts of the polysaccharides, saponins, flavonoids, alkaloids according to different dissolubility in different solvents. To identify them by using color reaction between chemical group and the reagent.Univariate and multi-factor orthogonal experiment is used to optimize the way extracting the highest polysaccharide content. UV spectrophotometry is used to detect content of polysaccharide after phenol-concentrated sulfuric acid color reaction. Macroporous resin purified the saponins. UV spectrophotometric is used to assay saponin content after the vanillin acetic acid-perchloric acid color reaction when the standard substance is Astragaloside. After purification by the polyamide, total flavonoids and icariin is assayed by UV spectrophotometric when icariin is as standard substance.Resultsthe total protein content of the ultrasonic abstraction is higher than the boiling water on the same time (about2times). The polyacrylamide gel electrophoresis gray value of the ultrasound abstraction is bigger than boiling water. Be extracted by boiling or ultrasound,the protein content or the polyacrylamide gel electrophoresis gray value of30min is higher than the extraction of15min or45min. If you add centipedes at the beginning of boiling water, the protein content and polyacrylamide gel electrophoresis gray values are higher than later adding centipedes, and the earlier you join the centipedes, the more protein you get.Polysaccharides, saponins, flavonoids and alkaloids can be extracted at the same flowsheet, and the diagnosis response is positive.Polysaccharide parts of the orthogonal experiment process is that desintegrate the drug from herb to coarse powder, supply as much as9times water(volum vs drug weight), extracte twice under2450MHz, each abstraction procedure should be lasting on for60minuties. Polysaccharide content ranges between about2.04%to 2.82%. Saponins content after macroporous resin purification is0.0978%.The content of flavones after purified could be28%.ConclusionThe centipede do not have to be taken without decocted. The protein content or polyacrylamide gel electrophoresis gray values is higher when centipede is decocted with other flavors drug.Polysaccharides, saponins, flavonoids and alkaloids can be extracted from the compound. The content should be improved further.