Effects Of Osteopontin On TIMP-1and TIMP-2Expression Of Chondrocytes In Hunman Knee With Osteoarthritis

Objective:To observe the effects of osteopontin (OPN) in different level on Tissue inhibitor of metalloproteinase (TIMP)-1mRNA and Tissue inhibitor of metalloproteinase(TIMP)-2mRNA expression in the chondrocytes of human knee with osteoarthritis.Methods:The study was performed on16specimens of cartilage obtained from osteoarthritic patients who underment total knee arthroplasty surgery. Then all the cartilage samples were cultured and purified to obtain chondrocytes in vitro,take the first passaged cells into three groups:(1)the control group,(2)treated with100ng/ml OPN for48h,(3)treated with1μg/ml OPN for48h, We designed and validated the real-time quantitative polymerase chain reaction (Q-PCR) assays with SYBR Green dyes for simultaneous quantification of the expression of the TIMP-1mRNA and TIMP-2mRNA. All results statistics were analysed by SPSS19.0.Results:1Compared to controls, after OPN(100ng/ml) interferenced48h, the TIMP-1mRNA expression in human OA chondrocytes, showed no difference of statistically significant.(p>0.05).2Compared to controls, after OPN(1μg/ml) interferencsd48h, up-regulated TIMP-1mRNA expression in human OA chondrocytes, and the difference showed statistically significant.(p<0.05).3The difference of TIMP-1mRNA expression between OPN (100ng/ml) and OPN (1μg/ml) interferenced in human OA chondrocytes showed statistically significant.(p<0.05). 4Compared to controls, after OPN(100ng/ml) interferenced48h, the TIMP-2mRNA expression in human OA chondrocytes, showed no difference of statistically significant.(p>0.05).5Compared to controls, after OPN(1μg/ml) interferencsd48h, up-regulated TIMP-2mRNA expression in human OA chondrocytes, and the difference showed statistically significant.(p<0.05).6The difference of TIMP-2mRNA expression between OPN (100ng/ml) and OPN (1μg/ml) interferenced in human OA chondrocytes showed statistically significant.(p<0.05).Conclusion:1OPN (100ng/ml) can not effect TIMP-1and TIMP-2mRNA expression in human OA chondrocytes.2OPN (1μg/ml) can up-regulate TIMP-1and TIMP-2mRNA expression in human OA chondrocytes.3The effect of OPN on TIMP-1/-2mRNA expression in hunman OA chondrocytes can change with the difference of level.