| ObjectiveTo study the therapy effect of exercise and ultrasound on articular cartilage of Osteoarthritis model of Rabbits, and the influence on cell apoptosis, bcl-2and MMp-13.MethodsCheck26healthy adult rabbits weighing roughly equal。Use random number table and select6as the Normal (A group), the remaining20making use of intra-articular injection of OA injury model. After the success of the model, according to the former method were randomly divided into B no treatment、C Exercise treatment、D ultrasound treatment. C Exercise group run on the Weight loss readmill every day for30minutes; D ultrasound treatment group treated by10minutes to give the lower intensity of ultrasound treatment. The three groups were treated28days. All rabbits were measured the weight and the range of motion before model、after model and after treatment. After treatment all the rabbits were treated with the death method of air embolism, executed the operated joint capsule incision exposed articular and observed articular cartilage injure healing. Take the modeling side of the articular cartilage and Synovial tissue after being observed, the articular cartilage fixed, decalcified, embedded, sliced. Observation the changes of HE staining and immunohistochemstry staining. comparing the quantity、morphology of articular cartilage cells and the expression bcl-2and MMP-13. Using the method of TUNEL to detect the apoptosis of chondrocyte and calculated the apoptotic rate. Synovial tissue fixed, embedded, sliced Observation the changes of HE staining and scored.Result1.The treated groups had great changes in the recovering of articular cartilage and range of motion (P<0.05)2.Inflammatory response of normal group of synovitis is mild, but it is obvious in synovitis of the model group.After ultrasound treatment and exercise therapy, inflammatory response of synovitis reduced. Compared with normal group (P<0.05), model group synovial score is high; Compared with the model group,ultrasound group、exercise group synovial score is lower(P<0.05); ultrasound group and exercise group were no significant differences (P>0.05).3.The TUNEL-positive cells can be seen in articular cartilage of each group. Comparing with the model group, the treated groups showed little TUNEL-positive cells (P<0.05); Comparing with the blank group, the model group showed more TUNEL-positive cells (P<0.05). But there were no statistical difference between the exercise group and the ultrasound treated group (P>0.05) 4.Immunohistochemistry staining showed little Bcl-2cell in the Normal group, but showed very little Bcl-2cells in the model group. Comparing with the model group, the treated groups showed many Bcl-2cells (P<0.05. But there were no statistical difference between the exercise group and the ultrasound treated group (P>0.05).5..Immunohistochemistry staining showed very little MMP-13cell in the normal group, but showed many MMP-13cells in the model group. Comparing with the model group, the treated groups showed little MMP-13cells (P<0.05). But there were no statistical difference between the exercise group and the ultrasound treated group (P>0.05).Conclusion:1. Bcl-2expression decreased, cell apoptosis increased, MMP-13expresstion increased in the OA mode.2.The ultrasound therapy and exercise treatment could reducing the apoptosis of articular cartilage by the method of increasing the expression of bcl-23. Reducing Inflammatory response of synovitis and articular cartilage by the method of lowering the expression of MMP-13, may be one of the mechanisms of exercise therapy、ultrasound treatment of osteoarthritis.4.Both exercise treatment and the ultrasound treatment could improve the repair of injury articular cartilages. |
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