The Correlation Of Expression Of FHIT With Survivin Protein And Prognosis In Patients With Cholangio-carcinoma

Background：Cholangio-carcinoma (carcinoma of bile duct) refers to a malignanttumor derived from bile duct epithelial cells, which occurred in the extrahepatic bileduct from left or right hepatic duct to the bile duct, but in recentyears, intrahepatic cholangio-carcinoma （intrahepatic cholangio-carcinoma, ICC)also under the scope of cholangio-carcinoma. According to location of the tumor,Cholangio-carcinoma can be divided into the extra-hepatic bile ductcarcinoma and intrahepatic cholangio-carcinoma. With the improvement of medicaldiagnostic technology, cholangio-carcinoma is common bile duct cancer with poorprognosis. Surgical resection or liver transplantation may be effective means forthe treatment of cholangio-carcinoma, but the vast majority of patients withcholangio-carcinoma miss the best closing timing of surgery, soto explore the molecular biological mechanisms ofcholangio-carcinoma and to find the non-surgical treatment of bile duct cancerhave became new hot spot. Currently the mechanism of molecularbiology of cholangio-carcinoma is unclear. The histological specimens of bile ductcancer were studied on the paper, FHIT and Survivin protein in the expressionof cholangio-carcinoma and adjacent normal bile duct tissue were contrasted, andanalysis of the clinical significance of FHITand Survivin protein and significance of prognosis.Objective: In this study, adjacent normal bile duct tissue andcholangio-carcinoma tissues were collected. To explore the correlation of expression ofFHIT and Survivin protein, evaluate its clinicopathological significance and overall survival time in Chinese patients with cholangio-carcinoma.Method:57cases of cholangio-carcinoma and the availability of23cases of adjacentnormal bile duct tissues were made into wax of slice, The immunohistochemicalanalysis was used to analyze the FHIT and Survivin expression in57cases ofcholangio-carcinoma and23cases of adjacent noncancerous normal bile duct tissues.Statistical analysis was used to evaluate the expression of FHIT and Survivin inrelation to the clinicopathological features and prognosis by chi-square test analysis,Kaplan-Meier survival analysis and COX multivariate regression analysis ofSPSS13.0software.Results: In the study, the result showed that the FHIT expression incholangio-carcinoma was lower than the expression in adjacent noncancerous normalbile duct tissues (P<0.05). Compared with the adjacent noncancerous, the expressionof Survivin was significantly increased in cholangio-carcinoma (P<0.05). Theexpression of FHIT was not related with age, gender, smoking,tumor site,HBV, andlymphnode metastas(P>0.05), but related with TNM stage and differentiation(P<0.05). The expression of Survivin were not found to be linked with age, gender,smoking, tumor site and HBV, but linked with TNM stage, differentiation andlymphnode metastas(P<0.05). There was inversely correlationon bewteen theexpression of FHIT and Survivin(P<0.05, r=-0.381). Basis on FHIT expression, thedata showed that there were a Statistically difference about the overall survival time bykaplan-Meier analysis(log rank=22.820,P=0.000). On the basis of Survivn expression,the Statistically difference was aslo found (log rank=16.535,P=0.000). By Coxregression analysis, the FHIT expression and Survivin expression were theindependent prognostic factors (P<0.05).Conclusion:The FHIT may be inversely correlated with the expression of Survivin inhuman cholangio-carcinoma tissues.It is suggested that loss of FHIT protein and overexpression of Survivin protein in human cholangio-carcinoma tissues hadcorrelated with the carcinogenesis and development of cholangio-carcinoma.the FHITexpression and Survivin expression may be the independent prognostic factors forcholangio-carcinoma.