| Background Friendenstein found that bone marrow cells which raised in petri dishessuch as plastic can to grow into fat cells, muscle cells, bone and cartilage cells in certainconditions, and these cells can maintain the multiplex differentiation potential even after20~30generations. The bone marrow stem cells could be differentiated to a variety ofsources of mesenchymal cells known as the bone marrow mesenchymal stem cells. Inrecent years, some studies found BMMSCs not only has the multiplex differentiationpotential, but also with low immunogenicity and easy to expand in vitro and othercharacteristics, so BMMSCs become to the ideal cells in tissue engineering. At present,replacement therapies of BMMSCs are mainly in two ways: by injecting BMMSCs intolocal vasculature and into damaged tissue，Among them, the former is moreprevalent.The injected BMMSCs migrate across the capillary endothelium，as the firststep of a tissue regenerating process．Previous findings have characterized the capabilityof BMMSCs of homing to ischemic or injured tissue，in the process of which adhesionmolecules was supposed to play a very important role.In experiments performed here，we studied the expression of adhesion molecules of rat BMMSCs which have beenstumilated by IL-1β and the mechanism of their adhesion to vascular endothelial cellswhich had not been clearly understood,in order to seeking a effective method topromote BMMSCs home to myocardial infarction tissue, and I hope this can provide a help for clinique.Objective To observe the effect of IL-1β that stimulate bone marrow mesenchymalstem cell to express adhesive molecular and home to the myocardial infarction tissue inrats.Methods The primary BMMSCs were isolated from rat bone marrow and cultured invitro and cells at passage and later were used in the following experiments:①BMMSCswere exposed to10μg/L IL-1β for24h，analyses of expression of VCAM-1，ICAM-1on cell surface were performed using flow cytometry;②Male SD rats were subjected toocclusion of left anterior descending artery,5-bromodeoxyuridine(Brdu) labeledMSCs,previously treated with10μg/L IL-1β were injected by intraveineuse reste24hafter myocardial infarction；the animals were executed another24h later, myocardialtissue was harvested and made into frozen section；the number of BMMSCs wascounted under a fluorescent microscope．Results Cells cultured in vitro adhered obviously after24h,cells were many kinds ofshape such as round、fusiform、and irregularity;after passages,the form of cells wasbecoming to be conformity,being fusiform.The reproductive activity of MSCs culturedin vitro was strong, BMMSCs grew colony and the shape of cell colony was on“swirl”;In physiological state,rat BMMSCs express high level of ICAM-1，while the expressionof VCAM-1was low;stimulated with10μg/L IL-1β the expression of VCAM-1increased concentration-dependently,while the expression of ICAM-1did not change markedly;more BMMSCs rested in myocardial tissue of the rat，compared to the controlgroups,when the stem cells pretreated with10μg/L IL-1β.Conclusion IL-1β can increase the expression of VCAM-1and can not effectthe expression of ICAM-1on rat BMMSCs; IL-1β can promote BMMSCs adhere toendothelial cells，promote BMMSCs migrate to the myocardial tissue．The mechanismmaybe is concerned with increase of VCAM-1. |
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