The Express Of Indexes About Migration And Chemotaxis Of T Cells In Acute Graft-versus-host Disease After Allogeneic Hematopoietic Stem Cell Transplantation

[Objective]Acute graft-versus-host disease(aGVHD)remain to be one of the major,life-threatening complications occurring after allogeneic hematopoietic stem cell transplantation(allo-HSCT).The effector cells must migrate to its target issues,in which many chemokine receptor,integrin,selectin and adhesion molecules is involed in.The study was to explore the roles of these factors in a-GVHD and search the good index and therapeutic target whick can be used to early warning,dynamic monitoring and treating aGVHD。[Methods]The blood was collected from the patients underwent allo-HSCT in 100 days.The expression of surface antigen,such as CCRT,CD45R,CCR4, CCR9,CXCR3,CCR5,integrinβ7,CD25,selectin L and adhesion molecules CD11a/CD54 were analysed by using flow cytometry.The CD4⁺ cells was purified from peripheral blood with magnetic system,and the mRNA expression of sphingosine 1-phosphate receptor 1(S1P₁)and integrinβ7 were detected by using real-time PCR.The clinical data was collected and analysis between aGVHD group and without aGVHD group.[Results]The expression of CCR7,Tna(?)ve,T_CM,CCR4,CCR9,CCR5,integrinβ7,CD25,CD54 and S1P₁ were markly higher in aGVHD group than without aGVHD group.Integrinβ7 was gut specific migrating molecule which different from intestinal GVHD group,inflammatory diarrhea recipient group and inflammatory diarrhea volunteer group significantly.The expression of T_EMand T_TDwere markly lower in aGVHD group than without aGVHD group.The expression of CD45RA,CXCR3,CD11a and CD62L were no significant chang between aGVHD and without aGVHD groups.[Conclusion]The CCR7,CCR4,CCR9,CCR5,integrinβ7,CD25,CD54 and S1P₁ are up-regulated in aGVHD after allo-HSCT.These signals could be used as new therapeutic target point in treating aGVHD.