| Background:Allergic rhinitis Is inspired by the Inhaled allergens, its IgE-mediated Type I allergic diseases, the Pathogenesis is complex, Involving genetic, immune and environmental factors,Is a polygenic disease, Its occurrence based on differences in levels of many genes, But its Genetic to be determined. Th1/Th2balance theory is An important point of allergic rhinitis, Th2-type cytokines Play an important role in the Immune etiology of Allergic rhinitis. Th2cells Synthesis and secretion IL-4、IL-5、IL-10、IL-13Etc. Cytokines for major. STAT6gene(Signal transducer and activator of transcription6) is considered to be the most important genes in Allergic diseases, It is through the tyrosine kinase (JAK-STAT) signaling pathway to Mediated A variety of Interleukin-induced gene expression, Thus to initiate gene transcription. It plays a key role in IL-13, IL-4-mediated Th2-type cytokine response and differentiation of Th2, And plays an important role in the generation of IgE, The IgE also plays an important role in the The pathogenesis of AR. There has Single nucleotide polymorphisms in3’untranslated region of2964points of STAT6gene, caused by the G'A conversion,2964points Located in the3’URT side,3’URT regional impact STAT6gene expression and mRNA stability, Ultimately affect STAT6protein cycle expression. IL-13is Secreted by activated CD4+Th2cells, With its level increasing with the secretion of Th2cells. It is an important class of Th2cytokines, Can cause the Humoral immune response as IgE increased, and the Infiltration of Eosinophis, plays an important role In the pathogenesis of allergic diseases. IgE attached to the surface. Of mast cells and basophils,Combined receptors of the surface of these two cells, releases a large number of inflammatory mediators, Chemokines、Cytokines、Adhesion molecules, Causes allergic reactions. There is some reference value in the process of Mediated allergic reaction.Objective and Significance:There are lots of research on Different sites of STAT6gene at home and abroad, lots of extensive research on allergic diseases, Especially Asthma. However, there are differences of race and disease phenotype definition due to Each of the study object, Results are not identical. Quite a controversy. on the G2964A sites of allergic diseases, especially allergic rhinitis. In this study, PCR-RFLP technique, ELIASA method, Determination of in vitro test systems were used. Study of the relevance of signal transduction and activator of transcription STAT6gene+2964G/A polymorphism and Allergic rhinitis of Shandong Han population.Material and Methods:1Study object:According to the Diagnosis and treatment principles of allergic rhinitis (LanZhou2004), choose natural population of Shandong, Grouped according to uniform standards,104patients with AR and115healthy controls2STAT6G2964polymorphism detection.(1)Venous blood4ml (EDTA-Anticoagulant),Separation of serum. DNA was extracted By genomic DNA Extraction kit, PCR amplification and PCR amplification products were sequenced by Bio-Engineering Company. Then analysised the Gene polymorphism of the Sequencing results.(2) plasma IL-13level were determined by ELISA.(3) take the separated plasma Use the Mast detection system(Produced by Germany), TIgE and SIgE Positive allergens corresponding SIgE level which measured by skin prick test.(4) Statistical analysis:Process the data With the SPSS13.0statistical software.Results:1Significant differences in genotype were observed between AR group and normal control group. In AR group the frequency of GG in G2964A sites was higher than Control group (χ2=7.17, P=0.028;), the frequency of Allele G was higher than Control group (χ2=5.82, P=0.022)2For the same genotype, IL-13concentration in AR group was higher than that in control group. In the same group,IL-13concentration of G allele carriers was higher than that of control group(P<0.05).3There was no significant differences of TIgE concentration between AR group genotypes (P>0.05).4There was no statistically significant in SIgE of Dust mites and ragweed pollen between different genotypes of STAT6G2964A sites (P>0.05). Conclusion:STAT6gene2964G/A site is associated with Allergic rhinitis, this site may be an important candidate gene for AR, and can affect the plasma IL-13level... |
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