Effects Of Diallyl Disulfide On Apoptosis Of Human Leukemia K562Cells And Expression Of Bag-1and Bax

ObjectiveTo study the effects of diallyl dsulfide (DADS) on apoptosis of human leukemiaK562cells and possible mechanisms.Methods1.The morphologic changes of leukemia K562after DADS treatment wereobserved by AO/EB.2.DNA ladder was detected by agarose gelelectrophoresis.3.The expression of Bax and Bag-1mRNA was estimated by reverse transcri-ptionpolymerase chain reaction (RT-PCR)48hours after DADS treatment.Results1. AO/EB stain demonstrated that treatment of40mg/L DADS with K562cellsfor48h showed the characteristic morphological changes of apoptosis.DNA gel electrophoresis revealed that treatment of40mg/L DADS with K562cells for48h could produce an obvious DNA ladder.3.RT-PCR results exhibited that treatment of40mg/L DADS with K562cells for48h led to an increased Bax mRNA Expression and a decreased Bag-1mRNAexpression compared with the control group（p＜0.05）.Conclusion1. DADS can induce apoptosis of human leukemia K562cells, and the optimalconcentration is40mg/L. DADS has apoptosis-inducing effect on K562cell in vitro;2. The related molecular mechanism is that DADS can activate the mitochondriaapoptosis way through upregulate Bax expression and downregulate Bag-1expression.