Studies On Chemical Constituents And Procedure Total Flavonoids And Chlorogenic Acid From Lonicera Similes Hemsl

Lonicera similes Hemsl from Nanjiang, Sichuan are dry bud or the blooming flowers early. It belongs Lonicera macranthoides Hand-Mazz.,Lonicera hypoglauca Miq. and Lonicera conmse DC. of Caprifoliaceae plants. The use of which includes heat detoxification, cold wind scattered the effect of heat. It is mainly used for the treatment of carbuncle swollen boils, Houbi, erysipel as fever sera dysentery, Fengreganmao, incidence of diseases such as warm. hat Hemsl in <Sichuan Traditional Chinese medicine standards>,<Hunan Traditional Chinese medicine standards> and <Guizhou quality Traditional Chinese medicine standards> used as Flos Lonicerae widely is the main variety of Flos Lonicerae in Sichuan. In order to coordinate the Sichuan Chinese medicine administrative bureau topic "The quality research of Lonicera similes Hemsl which Lonicera’s mainstream varieties in Sichuan "and find the effective chemical constituents of Flos Lonicerae. we try to find the chemical constituents corresponding with its functions more.This issue had studied on the chemical constituents and procedure of total flavonoids and chlorogenic acid of Lonicera similes Hemsl. With EtOAc and Butanol’s parts which extracted from the80%alcohol extraction of Lonicera similes Hemsl, the structures of9known compounds were isolated and identified on the basis of various spectroscopic datas. The constituents obtained:amentoflavone(Ⅰ), luteolin(Ⅱ), rutin(Ⅲ), oleanolic acid(Ⅳ), chlorogenic acid(Ⅴ), aesculetin(Ⅵ), inositol(Ⅶ), glucose(Ⅷ), β-sitosterol(Ⅸ). Compound Ⅰ, Ⅲ, Ⅳ, Ⅵ, Ⅶ,Ⅷ were obtained from this medicinal plant for the first time.We reaserched the extraction and purification process of main active ingredients total flavonoids and chlorogenic acid. By the experiments, the orthogonal design was used with the content of flavonoids and chlorogenic acid in Lonicera similes Hemsl. The best extraction technology was16times amount of50%ethano,1.5h of reflux, and 2times of extraction. And the best purification conditions are:concentration of Lonicer similes Hemsl. extract0.04g/mL; pH1～2; flow rate1.5mL·min-1; and the flavonoids and chlorogenic acid was efficiently separated by gradient elution with asuccession of10%,50%,95%alcohol. Finally, the flavonoids and chlorogenic acid’ purity is39.02%、25.37%.