The Detection Of Interleukin-33and The Correlated Of Research Of Interleukin-33and Rheumatoid Arthritis

ObjectiveTo determine serum interleukin-33(IL-33)levels and the expression of IL-33messenger RNA(mRNA) in peripheral blood mononuclear cells (PBMCs) in patients with rheumatoid arthritis (RA), and investigate their associations with clinical parameters and its effection in the pathogenesis of RA.MethodsEighty patients with rheumatoid arthritis were selected, twenty patients with osteoarthritis(OA) and fifteen healthy controls were selected. The serum IL-33levels of the three groups were detected by enzyme-linked immunosorbent assay(ELISA). Thirty RA patients were randomly selected from these eighty patients. Ficoll density gradient centrifugation was used to separate PMBC from peripheral blood. After the PMBCs were cultured in incubator with37℃and5%CO2for2hours, the monocytes would adhere to the wall. The adhere monocytes were collected, and the levels of IL-33mRNA expression in PBMCs were measured by reverse transcription-polymerase chain reaction (RT-PCR). The correlation between serum IL-33levels and clinical features of patients with RA were analyzed. Mann-Whitney U test、χ2test and Spearman test were used for statistical analysis.Result1、RT-PCR:The expression of IL-33mRNA in RA patients (0.8632±0.058) were significantly higher than OA groups[(0.676±0.091), P<0.05] and the healthy controls[(0.635±0.148), P<0.05]. To detected the correlation between the expression levels of IL-33mRNA and the clinical indicators with Pearson test, it showed there were only significantly correlation with rheumatoid factor(RF)-IgM.2、ELISA:The levels of serum IL-33in RA patients[(149.930±72.446)pg/ml] was significantly higher than that of the OA patients[(14.21±7.52)pg/ml, P<0.01] and the healthy controls[(12.583±3.139) pg/ml, P<0.01]. The levels of serum IL-33in RA patients were related with anticyclic citrullinated peptide(ACCP), anti-mutated citrullinated vimentin (MCV), rheumatoid factor(RF)-IgA, rheumatoid factor(RF)-IgG and rheumatoid factor(RF)-IgM. The levels of serum IL-33in the positive ACCP, MCV, RF)-IgA,(RF)-IgG,(RF)-IgM groups were significantly higher than that of the negative groups. The expression of IL-33mRNA in RA patients (0.8632±0.058) were significantly higher than OA groups[(0.676±0.091), P<0.05] and the healthy controls[(0.635±0.148), P<0.05].Conclusion1、The levels of IL-33is abnormally elevated both in RA serum and PMBC, suggesting that IL-33plays a certain role in the pathogenesis of RA.2、IL-33is associated with the autoantibody of patients with RA, suggesting that IL-33plays a certain role in the process of autoantibody production, partial antibodies are indicators of unfavorable prognosis in RA, suggesting that IL-33might be one of the factors of unfavorable prognosis in RA.