| Research PurposeThe main purpose of this experiment is:TO study prescription Huangzhu Xinwei decoction treatment of bile reflux gastritis model rat gastric mucosal damage efficacy, and discusses the party control of bile reflux gastritis mechanism of action, for future research lay a good foundation.Research MethodThe first step:animal group:40health clean grade Wistar rats, bisexual and each half, were randomly number divided into4groups10rats in each group, namely normal group, model group, Chineuse traditional medicine group (HuangZhu Xinwei decoction group), western medicine group (Domperidone conrtol group).The second step:animal made mould and medicine:Reflux liquid was given to each group the means of watering stomach except for normal group according to15ml/kg avoirdupois,two times a day, continuous14days. Made in the week3start, Chinese traditional medicine group to give Huangzhu Xinwei Decoction, Western medicine group give Domperidone treatment, and at the same time, given the normal group and the model group physiological saline treatment comfort a day, two times a day, continuous14days.The third step:index test:four groups of mice were in the last24hours after filling the stomach fast, the water for12hours. With10%of the first chlorine aldehyde solution according to0.3ml/100g standard intraperitoneal injection of anesthesia, from the sword ventral midline line dividing ashdod, the portion of the greater curvature side cut open, with saline water clean stomach tissue, blot moisture, each mouse accurate say take two group of gastric mucosal tissue, each group of each100mg, will each group addition of suitable amount of physiological saline respectively dolly,3000turn centrifugal10minutes take clear liquid on, with double antibody step clip method ELISA test (ELISA) detection in stomach tissue PEG-2content and ET-1. Research Results1.Rats in general observationsNormal control group:rats can spirit, diet, the activity is normal, hair dense gloss, defecate and no black and forming. The other three group rats made after2weeks in die appear spirit is weak, anorexia, activity decreases, unresponsive, hair loses luster and in different degree of fall off, defecate rare pond, weight loss. After treatment with HuangZhu Xinwei Decoction, the rat’s spirit get better, eat increased, activities increased, weight gain.2. The effect of Huangzhu Xinwei Decoction on content of PGE-2(ng/g) in gastr ic mucosa of exper iment bile reflux gastr it is rats contentThe table1showed,compared with normal group, the content of PGE-2in model group was lower,which had a very significant difference (P<0.01); Compared with model group, the content of PGE-2in Huangzhu Xinwei Decoction was higher, which had a very significant difference (P<0.01); Compared with Domperidone control group, the content of PGE-2in Huangzhu Xinwei Decoction was higher, which had a very significant difference (P<0.01).3. The effect of Huangzhu Xinwei Decoction on content of ET-1(ng/g) i n gastr i c mucosa of exper i ment bile reflux gastritis rats contentThe table2showed, compared with normal group, the content of ET-1in model group was higher,which had a very significant difference (P<0.01); Compared with model group, the content of ET-1in Huangzhu Xinwei Decoction was lower, which had a very significant difference (P<0.01); Compared with Domperidone control group, the content of ET-1in Huangzhu Xinwei Decoction was lower, which had a very significant difference (P<0.01).ConclusionHuangZhu Xinwei Decoction had an exactly significant effect on treatment of bile reflux gastritis. The prescription by improving the rats in stomach tissue in the content of prostaglandin-2, stimulate secretion of mucous, increase blood flow to mucosa, promote gastric mucosa in repair and reduce inflammation media release, and may reduce the rats in stomach tissue endothelin1, the content of blood vessels to reduce shrinkage material gastric mucosa damage, improve microcirculation, from two aspects of the organization of the stomach to play to the protection and treatment effect, which embodies Chinese medicine treatment of diseases advantage. |
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