The Expression Of Integrinβ1, Matrix Metalloproteinase-2and Phosphatase Of Regenerating Liver-3in The Ectopic Endometrial Tissue Of Women With Endometriosis

Objective: This study was designed to detectexpression of Intβ1, MMP-2and PRL-3in ectopic endometrial, eutopicendometrium of EMS and control endometrium, to explore the role ofthese proteins in the development of EMS and provide the experimentaldata and theoretical basis for clinical diagnosis and treatment of EMS.Methods: The samples collected for this study ware30cases of ectopicendometrium,20cases of eutopic endometrium from patients with EMSand20cases of control endometrium from the area of non-tumor inhysterectomy uterus with uterine fibroids in the same period. All of thepatients had not been treated with the hormone and anti-endometriosismedication six months before operation. The expression of Intβ1,MMP-2and PRL-3was measured by immunohistochemistry. Image thePro6.0professional image analysis system was used for measuring thevalue of the mean optical density (MOD) in positive cells. The statisticalmethods were carried out by the SPSS17.0software and the significantlevel was defined as P<0.05. Results:1. The expression value of Intβ1inectopic endometrium was increased, compared with eutopic endometriumand control endometrium (P<0.01, P<0.01respectively). The expressionof Intβ1in eutopic endometrium and control endometrium had no significant difference (P>0.05).2. The expression value of MMP-2inEctopic endometrium was increased, compared with eutopicendometrium and control endometrium (P<0.01, P<0.01respectively).The expression of MMP-2in eutopic endometrium and controlendometrium had no significant difference (P>0.05).3. The expression ofPRL-3in ectopic endometrium was higher than that in eutopicendometrium and control endometrium (P<0.05, P<0.01respectively).The expression of PRL-3in eutopic endometrium was higher than thecontrol endometrium (P<0.01).4. There was no cyclical changes of Intβ1in ectopic endometrium and eutopic endometrium, but in the the controlendometrium the difference was significant (P<0.05).The expression ofMMP-2of secretory phase was higher than that of proliferative phase inectopic endometrium, P<0.05. The expression of MMP-2in secretoryphase was higher than that of proliferative phase in eutopic endometriumand control endometrium, P>0.05.The expression of PRL-3in the threegroups showed no cyclical changes.5. Within the ectopic endometriumgroup，according to the R-AFS staging，the expression of Intβ1in I~IIstage was lower than that in III~IV stage, and the difference wassignificant (P<0.05). So was MMP-2in ectopic endometrium group. TheOD means of PRL-3in I~II stage was higher than that in III~IV stage,and the difference was significant (P<0.05).6. In ectopic and eutopicendometriotic tissue, there was a statistically significant correlation between Intβ1and MMP-2, with respectively correlation coefficients ofr=0.412, P<0.05; r=0.446, P<0.05. In ectopic endometriotic tissue, therewas a statistically significant correlation between MMP-2and PRL-3,with correlation coefficients of0.382(P<0.05), There was no correlationbetween MMP-2and PRL-3expression in eutopic endometrium (P>0.05).There was no correlation between Intβ1and PRL-3expression in ectopicendometrium and eutopic endometrium (P>0.05for ectopic endometrium;P>0.05for eutopic endometrium).Conclusion:1.There was higherexpression of Intβ1, MMP-2and PRL-3in ectopic endometrium and ineutopic endometrium than control endometrium. Ectopic endometrialtissue had a stronger ability of adhesion, degradation, invasion andmetastasis, indicating a closely relation to the progression ofendometriosis. Further support the "eutopic endometrial decisiontheory".2. The cyclical expression of Intβ1and MMP-2in controlendometrium is due to hormones in the menstrual cycle. Intβ1had nocyclical expression in ectopic endometrium because its normalphysiological balancing effect was destroyed.The higher expression ofMMP-2in secretory phase of ectopic endometrium indicated that it had amore invasive in ectopic endometrium. The expression of PRL-3in thethree groups showed no cyclical changes.3. The expression of Intβ1andMMP-2in the R-AFS staging of ectopic endometrium indicated thehigher stage, the stronger ability of adhesion and invasive. PRL-3was mainly working in the EMS early stage.4. Intβ1combining for itsreceptor affect the Ras-MAPK signal transduction pathway and enhancedthe expression of MMP-2, and PRL-3can improve signal transductionpathway to enhance the expression of MMP-2.