Expression And Significance Of PRL-3 And RhoC In Nsclc

Recently, many studies have shown that phosphatase of regenerating liver family involves in tumorigenesis and development of various types of tumors and its members phosphatase of regenerating liver-3 (PRL–3) plays an important positive regulatory role in cell proliferation, adhesion, migration, invasion and metastasis of a variety of tumor. But the reports concerning about correlation of phosphatase of regenerating liver -3 expression and invasion as well as metastasis in different pathological processes of lung cancer are rarely.Ras homologue C (RhoC) belongs to Ras homologue subfamily of small-molecule G protein superfamily and it is a important molecule in the Rho signal transduction pathway. Many studies have shown that ras homologue C is highly expressed in many tumors and has a highlyrelationship with invasion and metastasis.Objective To investigate the expression of PRL-3 and RhoC proteins and whether they were relative with invasion and metastasis of non-small cell lung cancer(NSCLC); To investigate the function of PRL-3 and RhoC in the migration of A549 cell and the relationship between them, which may provide experimental evidences of the mechanism of PRL-3 and RhoC in tumorgenesis and tumor development.Methods Immunohistochemical staining was used to detect the expression of PRL - 3 and RhoC in NSCLC in 92 cases, and statistical methods were used to analyse statistical significances of its expression in different groups and the relationship between them,The PRL-3 antibody and RhoC antobody were respectively used to block the function of PRL-3 and RhoC; Wound healing assay was used to detect the migration of A549 cell and Reversed transcript-polymerase chain reaction (RT-PCR) was used to detect the expression levels of PRL-3 and RhoC.Results The positive rates of PRL-3 and RhoC expression in NSCLC were 69.6%and 73.9%, and the expression of PRL-3 and RhoC were correlated with TNM stage and lymphatic metastasis and pleural metastasis (P<0.01), and they were correlated with each other(r=0.754, P<0.001). The migration of A549 cell has decreased when the functions of PRL-3 and RhoC were blocked; The expression of RhoC had decreased after PRL-3 was blocked, while the expression of PRL-3 did not change.Conclusion The expression of PRL - 3 and RhoC is higher in the higher TNM stage and lymphatic metastasis and pleural metastasis cases, and closely correlates with each other in NSCLC, which suggests that PRL-3 and RhoC might be in the same signal pathway and PRL-3 might promote the distant metastasis of cancer cell by RhoC and downstream factors. PRL-3, RhoC might have the function of promoting the migration of A549 cell; PRL-3 might up-regulate the expression of RhoC; PRL-3 and RhoC might be in the same signal pathway and PRL-3 might promote the metastasis of lung cancer cell by RhoC and its downstream factors, but RhoC may not have the function of feedback regulating the expression of PRL-3.