Experimental Study On Gastrointestinal Function Of Simo Decoction In Rats

Objective：Adhesive intestinal obstruction usually occursafter abdominal operation and especially after those abdominal operationscoupled with peritonitis that always lead to intestinal function disorder such asabdominal distension, enteroparalysis and so on. Although there were a lot ofdrugs and a variety of methods for prevention of intestinal adhesion afterabdominal operation, none of them was absolutely efficient and could beaccepted by clinical application. Researches show that increasesing alvineperistalsis incipiently can prevent intestinal adhesion effectively.Thisexperimental study was carried out to observe the affection of gastrointestinalfunction in rats and mice by simo decoction and explore the effection as well asmechanism of simo decoction for the prevention of intestinal adhesion afterabdominal operation. Methods: There were54SD rats which consist of27female rats and27male rats, animal models were established and50rats weredivided into five groups stochastically, retaining4rats which consist of2female rats and2male rats for blank control.High, medium, and low doses ofsimo decoction were used for the three groups respectively, with clebopride aspositive control group and with distilled water as negative control group. Theserats were irrigated equivalent simo decoction or mixed liquor of clebopride ordistilled water at the same time and all of them were irrigated methyl cellulosephenol red solution after60minutes. And then, these rats were put to deathafter15minutes. Pigment residual quantity was admeasured by light densitymeasuring ratio method and the rates of gastric evacuation were calculated forevery group. Also, there were50mice which were divided into five groupsstochastically. High, medium, and low doses of simo decoction were used forthe three groups respectively, with clebopride as positive control group andwith distilled water as negative control group. These mice were irrigatedequivalent simo decoction or mixed liquor of clebopride or distilled water at the same time and all of them were irrigated50%ink to press0.3ml/10g. Thesemice were put to death after30minutes and the ink promote rates werecalculated for every group. Results: The rates of gastric evacuation of high,medium, and low doses of simo decoction groups，positive control group andnegative control group were63.93±5.82、68.32±6.25、66.50±4.10、68.82±2.53、58.87±3.02respectively. And the rates of gastric evacuation existing significantdifference between the medium doses of simo decoction group and the negativecontrol group as well as the low doses of simo decoction group and the negativecontrol group(P<0.05). The ink promote rates of high, medium, and low dosesof simo decoction groups，positive control group and negative control groupwere65.39±10.00、69.29±6.42、65.25±9.05、72.60±7.70、57.29±6.61respectively. The medium doses of simo decoction could increase the inkpromote rate observably and the ink promote rates existing significantdifference between the medium doses of simo decoction group and the negativecontrol group(P<0.05).Conclusion: The medium and low doses of simodecoction could increase the rate of gastric evacuation observably. The mediumdoses of simo decoction could increase the ink promote rate observably. Equaleffective dosage of simo decoction can significantly increase gastric emptyingand small intestine promotional function in rats and mice. It is reasonable toconsider that simo decoction in the early postoperative application cansignificantly promote intestinal peristalsis after abdominal operation andeffectively prevent intestinal adhesion as well as intestinal obstruction.