Expression And Significance Of EDG-1and CFL-1in Hepatocellular Carcinoma

Objective:In this study, according to analyzing the expressions of endothelial differentiation gene1(EDG-1) and cofilin-1(CFL-1) in hepatocellular carcinoma (HCC) and correlation between them, and in combination with clinicopathological data, explore the relationships between them and clinicopathological characteristics, especially the invasion and metastasis, and evaluate the prognostic significance.Methods:57specimens collected from patients with hepatocellular carcinoma who were confirmed by pathological test during surgery in hepato-cholecyst surgical department of The Second Xiangya Hospital (from from January of2005to December of2006) as experimental group, and another15specimens from Peritumoral hepatic tissues (≥3cm away from the tumor) as controlled group. The specimens were fixed in10%formalin, routinely dehydrated and embedded in Paraffin. The expressions of EDG-1and CFL-1were detected by immunohistochemistry S-P method, The expressions were retrospectively analyzed in57specimens from HCC tissues (experimental group) and15specimens from normal hepatic tissues (controlled group), and compared with clinicopathological data. Record the lifetime of each case after surgery by medical follow-ups and do survival analysis. The data was calculated by SPSS16.0statistics software.Result:1. The immunohistochemistry result shows that immunostaining of EDG-1was located at cytoplasm.31cases in experimental group were positive and the ratio is54.4%(31/57), significantly higher than13.3%(2/15) of controlled group (P<0.05). The staining of CFL-1was also located at cytoplasm.38cases in experimental group were positive and the ratio is66.7%(38/57), significantly higher than13.3%(2/15) of controlled group (P<0.05). There is positive correlation between the expression score of EDG-1and CFL-1in HCC tissues (P<0.05).2. The clinicopathological evaluation shows that both of the expressions of EDG-1and CFL-1were significantly higher in HCC cases with metastasis than without metastasis (P<0.05), and correlated with differentiation degree of HCC tissues (P<0.05). However, the expressions had no correlations with gender, age, hepatitis B, alpha fetoprotein(AFP), size of tumor, degree of cirrhosis, and cancer embolus(P>0.05).3. The lifetime (months) in group of EDG-1expressing [(-)～(+)] is19.04±7.07, while it is10.28±5.58in group of [(++)～(+++)], which exists statistical significance (P<0.05). The lifetime (months) in group of CFL-1expressing [(-)～(+)] is21.65±6.47, while it is11.23±4.69in group of [(++)～(+++)], which exists statistical significance (P<0.05).4. Cox regression analysis showed that metastasis, cancer embolus, differentiation degree, EDG-1expression and CFL-1expression were independent prognostic factors (P<0.05).Conclusions:1. EDG-1and CFL-1were highly expressed in HCC tissues.2. The expressions of EDG-1and CFL-1were correlated with metastasis and differentiation degree of tumors, prompting the relationship between it and metastasis and infiltration of HCC, which could be an indication to identify the malignant degree of HCC.3. There is correlation between the expressions of EDG-1and CFL-1in HCC tissues4. Expressive levels of EDG-1and CFL-1are likely to be valuable indications in evaluaing of the prognosis of patients with hepatocellular carcinoma.