| Object:In this study we investigated the expression of GDF-15and GDF-5in brain tissue following cerebral ischemic reperfusion in SD rat and whether they were affected by the Danghong injection.Method:(1) Animal Groups:Sixty SD rat were randomly divided into four groups:Normal Group (n=6), Sham-operated Group (n=6), Ischemia-Reperfusion Group(I/R) and the Danhong injection group. Sham-operated Group and I/R Group are divided into four subgroups (each group has six animals):6hours group, lday group,3days group,7days group.(2) Mode:Each subject except normal group was anesthetized with10%Chloral Hydrate (30mg/kg,ip), supine fixed on bench,then neck skin was disinfected, the left sternocleidomastoid was cut open. We separated the sternocleidomastoid and form throat muscle and acting out the common carotid artery(CCA), free left CCA, internal carotid artery (ICA) and external carotid artery (ECA), ligated the CCA and ECA, cliped the heart end of ICA by bulldog clamp, cut a hag in ECA and ICA biforked mouth quickly, The line with a ball end was drived slowly from CCA to ICA, and promoted18～20mm blocking all blood supply in middle cerebral artery (MCA). We tied the fixed line, sutured the subcutaneous tissue and skin. After ischemia2h, we pull out the line to the bifurcation of ICA and ECA. Reperfusion was accomplished. The animals were sacrifice by decapitation at correspondence time.(3) Histological analysis of tissue injury:staining with stained with haematoxylin and eosin haematoxylin and eosin (HE).(4) Immunohistochemical Methods.(5) Experimental results were analyzed with the statistic soft package of Spss15.0Result:(1)Staning with HE:The tissues of normal group and sham-operated group was integrate,but that of I/R were changed with gliocyte hyperplasia and hypertrophia, interstitial edema, cellular edema, gliocyte and neuron edema, neuron necrosis.(2)Immunohistochemical Methods:The normal group and sham-operated group was measured low GDF-15and GDF-5, We measured the overexpression of GDF-15and GDF-5at6hours after I/R, with peak expression at1days, but the expression of GDF-15and GDF-5appeared to decline by7days and there was significant difference between groups(p<0.05). The data presented herein provide a positive correlation between expression of GDF-15and expression of GDF-5(p<0.05).(3) Compared with Danhong injection group, ischemia-reperfusion (I/R) group showed severe damage such as neurons degeneration, necrosis, hyperplasia of gliocyte, which were most obviously seen in7d subgroup. The expressions of GDF-15and GDF-5was singnificantly increased in Danhong injection group comparing to I/R group(p<0.05).Conclusions:(1) We reported that the expression of GDF-15and GDF-5was increased after SD rat brain I/R injury and can exert neuroprotective effects. There was a positive correlation between expression of GDF-15and expression of GDF-5after SD rat brain I/R injury.(2) Danhong injection could exert neuroprotective effects by influening the expression of GDF-15and GDF-5after SD rat brain I/R injury. |
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