| Objective:To explore the expressions of the heat shock protein20(Hsp20) and GFAP,and the protecion of Danhong injection through the expressions of Hsp20and GFAP in rat brain tissue following cerebral ischemic reperfusion.Methods:A cerebral ischemia-reperfusion rat model was established through the middle cerebral artery occlusion(MCAO) method.112healthy male SD rats were randomly divided into:normal control group, sham operation group, ischemia-reperfusion(I/R) group and Danhong injection intervention group (DI/R). Ischemia-reperfusion(I/R) group and Danhong injection intervention group were sub-divided into cerebral infarction group and the immumohistochemistry group. rats were injected with Danong injection(8ml/kg,Qd) intraperitoneally the day before experiment in intervention group;Ischemia-reperfusion(I/R) group with the same dose of normal saline. Normal group was put to death dI/Rectly.sham group and cerebral infarction group were killed24hours later and TTC staining evaluated of infarct size in rats. The expressions of Hsp20and GFAP in brain tissue were detected by using immunohistochemistry technique at the6th hour,24th hour,48th hour,72th hour and7th day after I/R in ischemia-reperfusion(I/R) group and Danhong intervention group.Every rats were neurological deficit scores before killed. Experimental results were analyzed with Spss17.0.Results:(1)In addition to6hours of ischemia/reperfusion at different time points outside,the neurological deficit score of Danhong intervention group was better than that of ischemia/reperfusion group.with the increase in ischemia/reperfusion time,the neurological deficit score was decreased in Danhong group (p<0.05)(2)The cerebral infarct size of Danhong intervention group was significantly smaller than that of ischemia/reperfusion group (P<0.05) (3) HE staining:after ischemia/reperfusion injury,the number of nerve cells reduced,and necrosis increased. Danhong injection intervention group of neurons in the pathological damage was lighter than the same time point of ischemia/reperfusion group. with the increase in ischemia/reperfusion time,the pathological damage became lighter in Danhong group (p<0.05).(4) Immunohistochemistry:Hsp20expressed in neurons and glicytes kytoplasm, Hsp20also expressed in vascular endothelial cell,GFAP expressed in astrocytes kytoplasm. There were a few expressions of Hsp20and GFAP in both normal control group and sham operation group, and there was no significant difference between the two groups (P>0.05). Compared with normal control group and sham operation group, both of the levels of Hsp20and GFAP were evidently upregulated at the6th hour,24th hour,48th hour,72th hour and7th day,and peaked at the72th hour (p<0.05) respectively, then both downregulated gradually.Compared with ischemia/reperfusion group, levels of GFAP in Danhong intervention group were evidently decreased at the6th hour,24th hour,48th hour,72th hour and7th day (p<0.05) and levels of Hsp20upregulated, inversely.Conclusions:1.After ischemia-reperfusion injury,the levels of Hsp20and GFAP in ischemia-reperfusion rats were both evidently upregulated.2. Injury of brain tissue after ischemia-reperfusion in Danhong intervention group became moderate. In addition, Danhong may upregulated levels of Hsp20and downregulated levels of GFAP, inhibiting the excessive proliferation of astrocytes. |
