Huanglianjiedu Decoction Experimental Study On Prevention And Treatment Of Acute Cerebral Infarction Rat Gastrointestinal Dysfunction

Objectives: To investigate the effect and the mechanism of Huanglianjiedu decoction onGastrointestinal Dysfunction after acute cerebral infarction in Rats.Methods:100male Wistar rats of the middle cerebral artery occlusion(MCAO) model wasestablished with modified Zea-Longa thread-occlusion method.All the rate are dividedrandomly in six groups:①Normal group;②Sham operation group;③Cerebralinfarction(CI) group;④Cimetidine group;⑤High-dose Huanglianjiedu decoction;⑥Standard-dose Huanglianjiedu decoction; When the rat regained consciousness two hours,use the Longa5level neurological injury score to evaluate the model. And then six groupsdifferently are taken sodium chloride solution, TCM Huanglianjiedu decoction, Cimetidineprower. Then take②④⑤⑥divided into2subgroups separately.Results：1. Establishing mice model with middle cerebral artery occlusion successfully2. Biochemical test results：The contents of GAS, VIP, and MTL in blood of model groupwere significantly higher than the normal group, All the7d group are lower than the4d group.(p<0.01). The contents of GAS, VIP, and MTL in blood of all theintervention group were significantly lower than the same time model group, buthigher than the normal group,Every intervention group7d subgroup are lower than4dsubgroup. Efficacy in turn as High-dose Huanglianjiedu decoction>Cimetidinegroup> Standard-dose Huanglianjiedu, the difference has statistics significance.3. Pathological observation results:3.1. The result is readable with naked eye, In model group gastroenteric mucosa of the rathas the dots of ulceration and congestion.4d subgroup relatively severer than the7d subgroup.3.2. Light microscopy observation: By the light-microscope the model group was showninflammatory infiltration, hemorrhage, edema and mucosal ulceration, etc；The injurious ofthe gastrointestinal mucosa4d subgroup are serious than the7d subgroup. In allintervention groups is still visible gastrointestinal mucosa damage. But the extent issignificantly reduced compared with the model group in the same time.The order ofimprovement degree is High-dose Huanglianjiedu decoction>Cimetidine group>Standard-dose Huanglianjiedu3.3. Electronic microscopy observation: The injurious of the gastrointestinal mucosa4dsubgroup are serious than the7d subgroup. In all intervention groups is still visiblegastrointestinal mucosa damage. But the extent is significantly reduced compared with themodel group in the same time. The order is High-dose Huanglianjiedudecoction>Cimetidine group> Standard-dose Huanglianjiedu.Conclusion:Acute cerebral infarction can produce varying degrees of gastrointestinal dysfunction,changes in gastrointestinal hormone levels may be a potential mechanism of action;Huanglianjiedu decoction can partially interfere with acute cerebral infarction can lead togastrointestinal dysfunction, the presence of the efficacy and measurementclosely related.