Intervention Research Of Qingrejiedu Fang In Type2Diabetes Inflammatory Factors

There are two parts in this thesis:Experimental research and Clinical research.Experimental research:Object:To observe the regulating efficacy of Qingrejiedu Fang on inflammatory factors and NF-kappa B/I kappa B signaling pathways in type2diabetic rats. Methods:To establish type2diabetic obese rat model by injecting with low dose of STZ and high-fat diet feeding. Then the rats were randomly divided into model group, high dose of Chinese medicine, low dose of Chinese medicine, metformin group. Resistin, Leptin, IL-1, and the mRNA level of NF-kappa B and I kappa B were measured after the treatment. Result:Compared with the model group, the Chinese Medicines groups of serum resistin, leptin, IL-1, NF-κB mRNA content decreased significantly, the expression level of Ⅰ.kappa B was increased. There is no significantly difference between the two groups of traditional Chinese medicine group. There is an also significant difference between the metformin group and the TCM group. Conclusion:Qingrejiedu Fang can significantly reduce resistin, leptin, IL-1levels in the rat serum and inhibit the NF-kappa B/I kappa B signaling pathway, which in the rat pancreatic islet beta cells and skeletal muscle cells, delay the complications of diabetes, and lower blood sugar.Clinical research:Object:To observe the regulating efficacy of Qingrejiedu Fang on TNF-α, IL-6, which in the sermon of type2diabetic patience Methods:60Patients with type2diabetes mellitus were randomly classified into two groups, in TCM Chinese medicine group patient (n=30) were treated by Qingrejiedu Fang, Each200ml,2times a day and in control group patient (n=30) were treated by original western medicine,8weeks was a course of treatment. Compare serum TNF-alpha, IL-6level between the two groups and comprehensive evaluation of the efficacy. Result:Compared with the control group, serum TNF-alpha, IL-6levels in type2diabetes patients were significantly increased; Qingrejiedu Fang can significantly relieve patients’ symptoms and lower serum TNF-alpha, IL-6, glucose, glycosylated hemoglobin, fasting insulin index and insulin resistance index. Conclusion:Clinical observations suggest that Qingrejiedu Fang can significantly relieve the clinical symptoms of hot poison intrinsic and excessive heat of lung and stomach in type2diabetes, significantly reduced serum levels of inflammatory factors, relieving insulin resistance, have a good therapeutic effect on type2diabetes.