The Influence Of Transplantation Of HUCMSCs On Expression Of GAP-43and SPRR1A And Nerve Functions In Subacute Sci Rats

ObjectivesTo observe the effect of expression of GAP-43and SPRR1A gene in subacutespinal cord injury (SCI) rats which have been transplanted Human UmbilicalCord-derived Mesenchymal Stem Cells (HUCMSCs).And the benefits to recover thenerve function are also observed. To study the mechanism of HUCMSCs transplantationtreating subacute spinal cord injury.Methods1. Using modified Allen’s method to establish rats’T10SCI model. There are12rats in sham-operated group(only with Laminectomy,without SCI, without HUCMSCstransplantation) and20rats in injuried group(with Laminectomy,with SCI,withoutHUCMSCs transplantation).In the different transplant groups, there are20ratsin1×106stem cell transplant group(with Laminectomy,with SCI,with1×106HUCMSCstransplant),20rats in3×106stem cell transplant group(with Laminectomy,withSCI,with3×106HUCMSCs transplant),20rats in5×106stem cell transplant group(withLaminectomy,with SCI,with5×106HUCMSCs transplant).Then every group israndomly devided into1day group,7days group,14days group and21days group afterthe transplantation. Each group is devided into4sub-groups(the1st,7th,14thand21thdayafter transplant). There are3rats every sub-group in sham-operated group. There are5rats every sub-group in injuried group and each transplant group. In each transplantgroup, the HUCMSCs are injected through the rat tail vein after the14thday of thetransplantation.2. Valuing the improvement of movement function at the1st,7th,14th,21thday aftertransplant by BBB score method.3. Using immunohistochemical techniques to detect GAP-43SPRR1A gene expression at the1st,7th,14th,21thday after transplant. Using immunofluorescencestaining technique to trace the migration and survival of HUCMSCs in rats’ body atthe7th,14th,21thday after transplant. Then using microscope and electronic microscopeto observe constructive change of injuried spinal cord tissue at the21thday aftertransplant.Results1. Each transplant groups and injuried group have varying degrees of recoveryof movement function, the improvement of rats’ movement function at the14thday and21thday after the stem cell transplant have significant differences with injuried group (p<0.01). The3×106and5×106stem cell transplant group had the significantimprovement of movement function than1×106stem cell transplant group.2. On the7th,14th,21thday after transplant, the expression of GAP-43and SPRR1Agene in the area of SCI had shown that transplant groups are significant higher thaninjuried group(p <0.01); and the positive expression of genes of3×106and5×106stemcell transplant group are much higher than1×106(p <0.05).3. On the7th,14th,21thday after transplant, we can find that HUCMSCs gatheringat the injuried spinal cord area by immunofluorescence.4. On the21thday after transplant, comparing to injuried group, the construction ofspinal cord tissue in transplant groups are more complete. The3×106stem celltransplant group and5×106stem cell transplant group are more complete than1×106stem cell transplant group.ConlusionsIntravenous transplanting HUCMSCs is benefit to the constructive restoration ofinjuried spinal cord and the recovery of movement function.And HUCMSCs canmigrate to the injuried spinal cord, then survive and change the micro-enviroment ofinjuried spinal cord, and promote the expression of GAP-43and SPRR1A. Further more,3×106stem cell transplant is the best way to cure the SCI.