| Objective This topic is to study the expression of the Bax and Caspase-3after thesub-acute spinal cord injury on rats and investigate the effect on the neural function bymeans of transplantation human umbilical cord-drived mesenchymal stem cells（hUC-MSCs）.Method1.92healthy adult male SD rats were used to establish the model of the ratspinal cord (T10) injury with improved Allen’s attack method，Then they were randomlydivided into5groups: untreated group, pure SCI group, group a, group b and group c.For each group, it was divided into4sub-groups randomly: the1th day,7th day,14thday and21th day after transplantation. For untreated group, there were3adult male SDrats in each sub-group, while for the rest of groups there were5rats in each sub-group.After injured the14th days, except for the pure SCI group, other three transplantationgroups were intravenously injected HUC-MSCs cell saline suspension through the tailof rats (for group a,1x106MSCs; for group b,3x106MSCs; for group c,5x106MSCs).2. The1th day,7th day,14th day and21th day after the transplantation, the BBB scoresystem was adopted to evaluate the recovery situation of the rat movement functionbefore and after the treatment.3. The21th days after the transplantation, take a sample from each group to observe themorphological variation of the spinal cord injured organization.4. After the transplantation, at3different points of time (7days,14days,21days),immunofluorescence technique was used to observe the migration and survival of thehUC-MSCs.5. The immunohistochemical method was selected to check seperately the expression ofthe Bax and Caspase-3in the SCI rats at each point of time during1day,7days,14daysand21days.Result1. The movement function of rats from transplanted hUC-MSCs groups and pure SCI group (comparison group) were recovered at different degrees. Compared to thepure SCI group, the spinal cord structure at the injury area of the transplantedhUC-MSCs groups was completed relatively and indicated an obvious improvement ofthe movement function (P＜0.05).2. The immunofluorescence showed that the hUC-MSCs cell survived a lot andconcentrate at the injury area and its surrounding zone.3. The7th day,14th day and21th day after transplanted hUC-MSCs, theexpression of the Bax in the injury area of the spinal cord of transplanted hUC-MSCsgroups and its surrounding organization was obviously decreased compared to that ofthe pure SCI group (P＜0.05).4. The7th day,14th day and21th day after transplanted HUC-MSCs, theexpression of the Caspase-3in the injury area of the spinal cord of transplantedhUC-MSCs groups and its surrounding organization was obviously decreased comparedto that of the pure SCI group (P＜0.05).Conclusion1. hUC-MSCs can penetrate the blood brain barrier through the transplantation ofperipheral vascular and migrate, concentrate, survive and differentiate in the spinal cordinjured area and then promote the recovery of the neural function on the rat sub-acutespinal cord injury and it can decrease effectively the expression of Bax and Caspase-3inthe injured area and restrain apoptosis as well as avoid the secondary injury to play arole of protecting the neuron. |
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