Effects Of HUCMSCs On Mouse Dry Eye Disease Of Sj(?)gren Syndrome

Backgroud The current incidence of dry eye disease is gradually increased but the existing treatments can only relieve the patient’s symptoms, and effective therapy for dry eye disease is still researching.Objective We exploit a new strategy for treating dry sydrom by using human umbilical cord mesenchymal stem cells(HUCMSCs) and compare the treatment effect of different transplantation methods.Methods HUCMSCs were used to treat male NOD/Ltj mice aged at 24 week by tail intravenous injection, lacrimal injection and eye drops. Aqueous tear production was measured weekly by phenol red cotton thread. Defect degree of corneal epithelium were evaluated by corneal fluorescein staining. The levels of IL-6, IL-17, TNF-α, and IFN-γ in serum were examined by Elisa. The protein levels of P65, Stat3, Stat5 and Erk in lacrimal gland were detected by Western blot.Results Mice with tail vein injection and lacrimal injection have higher amounts of aqueous tear production significantly compared with those untreated(p<0.05), while mice with eye drops treatment have no significance improvement. Corneal epithelial defects were alleviated in the mice with treatment of tail vein injection and lacrimal injection. IL-6, IL-17, and TNF-α in serum were significant reduced and the expression of STAT3 and STAT5 were significantly decreased in the mice treated with tail vein injection and lacrimal injection.Conclusion Intravenous and lacrimal injections of HUCMSCs greatly alleviate the syndrome of dry eye disease by attenuating the inflammatory reaction,The method of eye drops may through lubricate and nutrition cornea to relieve dry eye.