The Protective Effect Of Proanthocyanidins On Reproductive Toxicity In Male Mice Induced By Trans Fatty Acids

Objective:1.To observe the reproductive toxicity of different dose of Trans Fatty Acids(TFAs) onmale mice.2.To preliminary investigate the protective effect and mechanism of proanth-ocyanidins(PC) on reproductive toxicity in male mice induced by TFA, and provideexperimental basis for evaluating the protective effect of PC on reproductive toxicity inducedby TFA.Methods:Taking TFA and PC as the test materal, ninety six healthy adult male Kunming(KM)mice were divided into eight groups randomly: control group, low-TFA group, middle-TFAgroup, high-TFA group, PC control group, L-TFA+PC group, M-TFA+PC group, H-TFA+PCgroup. The mice in low, middle and high dose of TFA exposed groups were induced byintragastric TFA25mg/kg,50mg/kg,100mg/kg respectively on the morning and equalamounts of saline at afternoon, every other day for ninety days. The mice in low, middle andhigh dose of TFA+PC groups were induced by intragastric TFA25mg/kg,50mg/kg,100mg/kg respectively on the morning and equal amounts of PC(100mg/kg) at afternoon,every other day for ninety days. The mice in control group and PC control group wereintragastric with saline and PC(100mg/kg), and all mice were intragastric with10ml/kg.bwvolumn liquid. The mice were sacrificed after experiment and taken blood, testis, epididymisand kidney for weighting and calculating the organ coefficient. Testicular tissue pathologicalmorphological changes of mice were observed by using routine pathological sections and HEstaining. Alteration of sperm quality were observed under optical microscope. The serumlevel of T, LH and FSH were tested by full automatic biochemical analyzer, and MDA, SODand GSH-PXenzyme activities were measured by reagent kit. Results:1. Compared with control group, the weight and net increase of weight of mice inmiddle-TFA and high-TFA group were signigicantly higher. The net increase of weight ofmice in H-TFA+PC group were decreased than the same dose of TFA exposed group (P＜0.05).2. The testis coefficient of mice in high-TFA group were lower than that of controlgroup. The testis coefficient of mice in H-TFA+PC group were decreased than the same doseof TFA exposed group (P＜0.05). The effect of PC on the organ coefficient of testis,epididimis and kidney was little in the other exposed groups, and the differences were notsignificantly(P＞0.05).3. Testicular pathology showed that the testis seminiferous tubule of mice in controlgroup was complete and epithelial cells was normal, and there were a lot of mature spermsaw in lumen. The basement membrane of testis tissue in TFA exposed groups wereambiguous, nucleus gap widened, with a portion of the canal wall deletion and no spermintraluminal. Given PC as a protective materal, testicular tissue morphology of mice inTFA+PC groups were improved than TFA exposed group, in which low dose and middledose of TFA groups showed significant improvement, which showed leydig narrows,decreased mesenchymal cells particles, increased seminiferous cell number, normalseminiferous epithelium cell level and more intraluminal sperm.4. Compared with the control group, spermcount, viability and motility of mice inmiddle-TFA and high-TFA exposed groups decreased and abnormal rate increased(P＜0.05).spermcount, viability and motility of mice in M-TFA-PC and H-TFA-PC group wereincreased to different extends, and deformity rate were reduced than the same dose of TFAexposed group, in which the difference was significant(P＜0.05). The deformity of spermwas mainly with angle, curved body and tail, folding body, not stereotypes anddouble-tailed.5. The serum level of T, LH and FSH in high-TFA group were lower than controlgroup(P＜0.05). The serum level of T, LH and FSH in H-TFA+PC group were increased indifferent degrees than high-TFA group, and there was significant difference(P＜0.05). 6. LDH-X and GGT activities in high-TFA group were lower than control group(P＜0.05). LDH-X and GGT activities in H-TFA+PC group were increased in different degreesthan high-TFA group, and there was significant difference(P＜0.05). The difference ofenzyme activities in the other group was not significantly(P＞0.05).7. Compared with control group, the content of MDA in testis of mice in middle-TFAand high-TFA group increased, SOD and GSH-PXenzyme activities decreased(P＜0.05).SOD, GSH-PXand CAT activities in H-TFA+PC group were increased than high-TFAgroup(P＜0.05). GSH-PXactivities in M-TFA+PC group were increased than middle-TFAgroup(P＜0.05). The enzyme activities and oxidation products MDA were not foundsignificant differences in other groups(P＞0.05).Conclusions:1. Different doses of TFA could cause reproductive system injury in subchronicpoisoned male mice, and high doses of TFA had severe injury on reproductive function.2. PC(100mg/kg．bw) can repair the damage effect of reproductive system of male miceinduced by TFA.3. The antioxidant effect was one of the important mechanisms of PC protection(repair)effect on male reproductive function induced by TFA.