| Objective:In the last few years, Phthalic Acid Esters(phthalates, PAEs) is wildly used asindustrial chemical plasticizer all over the world. Dibutyl Phthalate(Dibutyl phthalate,DBP) is a common synthetic organics in PAEs which has been used in production andprocessing of resin, rubber, PVC and other products, and also used in paints, coatings,inks and other industries. DBP’s market is in great demand, and with regards that it isubiquitous in the environment, people have widespread concern about the safety ofDBP. This research is aim to irrigate and expose the mice and observe their changesduring14d and28d to investigate DBP’s influence on male reproductive function incase to provide basis for safety of plasticizer.Method:40health male ICR mice are randomized into control group and200、400、800mg·kg-1DBP group. Each group holds10mice which have been continuouslyirrigated and afforded DBP, dosages are0、200、400and800mg·kg-1·d-1, the controlgroup only afforded corn oil. Then we execute20mice on2weeks and4weeks afterexposure, taking their testicles and observing LDH、SDH、G-6-PD, SOD、MDA、GSH-Px, pathologicalteration, cell cycle and apoptosis and other lesion of testiculartissue.Result:1. Changes in mice weight and testis organ coefficient2weeks after exposure, weight and testis organ coefficient of each exposuregroup have no obviously changes(P>0.05),4weeks later, weight of800mg·kg-1DBPgroup is obviously higher than control group and200mg·kg-1DBP(P<0.05), testisorgan coefficient of400、800mg·kg-1DBP are obviously lower than control group and200mg·kg-1DBP(P<0.05).2. Influence on Mould Activity of testicular tissue 2weeks after exposure, LDH activity have no obvious changes (P>0.05),G-6-PD activity of800mg·kg-1DBP is obviously higher than control group and200、400mg·kg-1DBP group (P<0.05), SDH activity of400、800mg·kg-1DBP are obviouslylower than control group and200mg·kg-1DBP group (P<0.05);4weeks after exposure,LDH activity of400、800mg·kg-1DBP group are obviously lower than control groupand200mg·kg-1DBP group (P<0.05), G-6-PD activity of800mg·kg-1DBP is obviouslyhigher than control group and other exposure group (P<0.05), and G-6-PD activity of200mg·kg-1DBP group is obviously lower than control group and other exposuregroup (P<0.05), SDH activity of all exposure group are obviously lower than controlgroup (P<0.05),400、800mg·kg-1DBP group are obviously higher than200mg·kg-1DBP group (P<0.05), and800mg·kg-1DBP group is obviously higher than400mg·kg-1DBP group (P<0.05).3. Influence on Lipid Peroxidation of testicular tissue2weeks after exposure, MDA of400、800mg·kg-1DBP group are obviouslyhigher than control group and200mg·kg-1DBP group.200mg·kg-1DBP group isobviously lower than control group (P<0.05), MDA of800mg·kg-1DBP group isobviously higher than400mg·kg-1DBP group (P<0.05). SOD activity of200mg·kg-1DBP group is obviously higher than control group (P<0.05), SOD activityof800mg·kg-1DBP is obviously lower than control group (P<0.05), SOD activity of400、800mg·kg-1DBP group is obviously lower than200mg·kg-1DBP group (P<0.05),GSH-Px activity of all exposure group are lower than control group (P<0.05), andthere is no obvious difference among all exposure group (P>0.05);4weeks afterexposure, MDA of200mg·kg-1DBP group is obviously lower than control group(P<0.05), MDA of400、800mg·kg-1DBP group are obviously higher than controlgroup and200mg·kg-1DBP group (P<0.05), and MDA of800mg·kg-1DBP group isobviously higher than400mg·kg-1DBP (P<0.05), SOD activity of800mg·kg-1DBPgroup is obviously lower than200、400mg·kg-1DBP (P<0.05), GSH-Px activity of allexposure are obviously higher than control group (P<0.05), and GSH-Px activity of400mg·kg-1DBP group is obviously higher than200、800mg·kg-1DBP group (P<0.05).4. Influence on Relative Count and Sperm Aberration Rate of testicular tissue2weeks after exposure, mice sperm count of400、800mg·kg-1DBP group areobviously lower than control group and200mg·kg-1DBP group (P<0.05). Sperm Aberration Rate of800mg·kg-1DBP group is obviously higher than control group andother exposure group (P<0.05), and400mg·kg-1DBP group is obviously higher thancontrol group and200mg·kg-1DBP group (P<0.05);4weeks after exposure, micesperm count of400、800mg·kg-1DBP group are obviously lower than control groupand200mg·kg-1DBP group (P<0.05), Sperm Aberration Rate of800mg·kg-1DBPgroup is obviously higher than control group and other exposure group (P<0.05),400mg·kg-1DBP group is obviously higher than control group and200mg·kg-1DBPgroup (P<0.05), and there is no obvious difference among control group and200mg·kg-1DBP group (P>0.05).5. Influence on Cell Cycle and Cell Apoptosis of testicular tissue2weeks after exposure,G0/G1Period of each exposure group are obviouslyhigher than control group (P<0.05),400、800mg·kg-1DBP group are obviously higherthan200mg·kg-1DBP group (P<0.05), S Period of400mg·kg-1DBP group is obviouslylower than control group(P<0.05), S Period of800mg·kg-1DBP group is obviouslylower than control group and200mg·kg-1DBP group (P<0.05), G2/M Period of400、800mg·kg-1DBP group are obviously lower than control group and200mg·kg-1DBPgroup (P<0.05), testis cell apoptosis rate of each exposure group are obviously higherthan control group (P<0.05).4weeks after exposure,G0/G1Period of400、800mg·kg-1DBP group areobviously higher than control group and200mg·kg-1DBP group (P<0.05), S Period of400mg·kg-1DBP group is obviously lower than control group and200mg·kg-1DBPgroup (P<0.05), S Period of800mg·kg-1DBP group is obviously lower than any othergroup (P<0.05), G2/M Period of400、800mg·kg-1DBP group are obviously lower thancontrol group and200mg·kg-1DBP group (P<0.05); testis cell apoptosis rate of eachexposure group are obviously higher than control group (P<0.05), and800mg·kg-1DBP group is obviously higher than200、400mg·kg-1DBP group (P<0.05).6.Pathological Examination Result of testicular tissue2weeks and4weeks after exposure, each exposure group has different degreesof changes such as incomplete cell membrane, cell gap, seminiferous epitheliumshedding, decrease in the number of sperm in cell tube.ConclusionThe research shows that DBP can cause decrease of LDH activity of male mice testicular tissue, MDA level, can disturb activity of G-6-PD、SDH、SOD、GSH-Px,and can decrease amount of sperms and increase testis cell apoptosis rate. Meanwhile,DBP can influence mice cellular processes, make G0/G1retardant and partly causecell apoptosis. |
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